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Chemokine Detection Using Receptors Immobilized on an SPR Sensor Surface

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Rodríguez Frade, José Miguel and Martínez Muñoz, Laura and Villares, Ricardo and Cascio, Graciela and Lucas, Pilar and Pérez Gomáriz, Rosa María and Mellado, Mario (2016) Chemokine Detection Using Receptors Immobilized on an SPR Sensor Surface. In Chemokines. Methods in enzymology, 570 (570). Elsevier, Netherlands, pp. 1-18. ISBN 9780128021712 (Hardcover) 9780128021958 (ebook)

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Official URL: http://www.sciencedirect.com/science/article/pii/S0076687915005170



Abstract

Chemokines and their receptors take part in many physiological and pathological processes, and their dysregulated expression is linked to chronic inflammatory and autoimmune diseases, immunodeficiencies, and cancer. The chemokine receptors, members of the G protein-coupled receptor family, are integral membrane proteins, with seven-transmembrane domains that bind the chemokines and transmit signals through GTP-binding proteins. Many assays used to study the structure, conformation, or activation mechanism of these receptors are based on ligand-binding measurement,as are techniques to detect new agonists and antagonists that modulate chemokine function. Such methods require labeling of the chemokine and/or its receptor, whichcan alter their binding characteristics. Surface plasmon resonance (SPR) is a powerful technique for analysis of the interaction between immobilized receptors and ligands in solution, in real time, and without labeling. SPR measurements nonetheless require expression and purification steps that can alter the conformation, stability, and function of the chemokine and/or the chemokine receptor. In this review, we focus on distinct methods to immobilize chemokine receptors on the surface of an optical biosensor. We expose the advantages and disadvantages of different protocols used and describe in detail the method to retain viral particles as receptor carriers that can be used for SPR determinations.


Item Type:Book Section
Uncontrolled Keywords:Chemokines; Chemokine receptors; Surface plasmon resonance; Viral particles
Subjects:Medical sciences > Biology > Biochemistry
ID Code:41286
Deposited On:14 Feb 2017 09:40
Last Modified:14 Feb 2017 09:54

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