Publication: Efectos de un extracto de "Fraxinus excelsior L." en ratas espontáneamente hipertensas
Loading...
Files
Official URL
Full text at PDC
Publication Date
2017-03-01
Authors
Advisors (or tutors)
Editors
Journal Title
Journal ISSN
Volume Title
Publisher
Universidad Complutense de Madrid
Citations
Exportar
Abstract
Los fitoquímicos son sustancias con actividad biológica que se encuentran en las plantas. Estos compuestos pueden incluirse en alimentos que se denominan alimentos funcionales, porque mejoran la salud de los consumidores. Algunos de estos alimentos se utilizan para la prevención y/o el tratamiento de la hipertensión, una patología clave en el desarrollo de las enfermedades cardiovasculares. En la presente Tesis Doctoral, hemos estudiado el efecto de un extracto de fresno común, denominado FraxiPure (FRP), que contiene fitoquímicos terpénicos y fenólicos, sobre la presión arterial de ratas espontáneamente hipertensas (SHR). Estos animales son un modelo experimental de hipertensión muy utilizado, ya que el desarrollo de la hipertensión en ellos presenta muchas similitudes con el desarrollo de la hipertensión esencial en humanos. Se administraron, por vía intragástrica, distintas dosis de FRP (5, 10, 20, 30 y 40 mg/kg), disueltas en 1 ml de agua, a ratas SHR con 17-20 semanas de vida, que pesaban 310-320 g, y se evaluaron las correspondientes modificaciones de la presión arterial. Para estos ensayos, se usó agua como control negativo, y 50 mg/kg de Captopril como control positivo. Se midió la presión arterial sistólica (PAS) y diastólica (PAD) de las ratas, mediante la técnica del manguito en la cola, antes de la administración, y también 2, 4, 6, 8, 24 y 48 horas post-administración. FRP disminuyó la presión arterial de las ratas, aunque menos que Captopril, y la disminución máxima de la PAS que ocasionó este extracto (-26,88 ± 3,05 mm Hg) la observamos 4 horas después de administrar 20 mg/kg del mismo. Esta dosis del extracto no modificó sin embargo la PAS de las ratas Wistar-Kyoto, que son el control normotenso de las ratas SHR. FRP también disminuyó la PAD de las ratas SHR, y la disminución máxima de esta variable se observó 4 horas después de administrar 30 mg/kg del extracto (-39,16 ± 12,1 mm Hg)...
Phytochemicals are biologically active compounds that are present in plants. They may be included in functional foods that improve health of consumers. Some of these foods are used for the prevention and/or the treatment of hypertension, a key pathology in the development of cardiovascular diseases. In this Doctoral Thesis, we have studied the effect of a Fraxinus excelsior L. seed extract, named FraxiPure (FRP), that contains terpene and phenolic phytochemicals, on spontaneously hypertensive rats (SHR). These animals are a well known experimental model for hypertension, because the development of this disease in this strain is very similar to the development of essential hypertension in humans. Different doses of FRP (5, 10, 20, 30 and 40 mg/kg), always dissolved in 1 ml of water, were administered, by intragastric intubation, to 17-22-weekold male SHR (300-350 g). Distilled water was used as negative control and 50 mg/kg Captopril was used as positive control. The systolic blood pressure (SBP) and the diastolic blood pressure (DBP) were recorded by the tail cuff method in the rats, before administration and also at different moments postadministration (2, 4, 6, 8, 24 and 48 hours). Captopril decreased arterial blood pressure in the SHR. FRP also decreased arterial blood pressure in these animals, but the effect of this extract was always less accentuated than the effect of Captopril. The most effective dose of FRP to decrease SBP in the SHR was 20 mg/kg. This dose of FRP caused in this strain the maximum decrease in SBP (-26.88 ± 3.05 mm Hg) 4 hours post-intragastric administration. Nevertheless, the same dose of the extract did not modify SBP in Wistar-Kyoto rats, that are considered as the normotensive control for the SHR. FRP decreased also DBP in the SHR. The maximum decrease in this variable (-39.16 ± 12.1 mm Hg) was observed 4 hours postadministration of 30 mg/kg FRP. Surprisingly, higher doses of FRP decreased less SBP and DBP in the SHR...
Phytochemicals are biologically active compounds that are present in plants. They may be included in functional foods that improve health of consumers. Some of these foods are used for the prevention and/or the treatment of hypertension, a key pathology in the development of cardiovascular diseases. In this Doctoral Thesis, we have studied the effect of a Fraxinus excelsior L. seed extract, named FraxiPure (FRP), that contains terpene and phenolic phytochemicals, on spontaneously hypertensive rats (SHR). These animals are a well known experimental model for hypertension, because the development of this disease in this strain is very similar to the development of essential hypertension in humans. Different doses of FRP (5, 10, 20, 30 and 40 mg/kg), always dissolved in 1 ml of water, were administered, by intragastric intubation, to 17-22-weekold male SHR (300-350 g). Distilled water was used as negative control and 50 mg/kg Captopril was used as positive control. The systolic blood pressure (SBP) and the diastolic blood pressure (DBP) were recorded by the tail cuff method in the rats, before administration and also at different moments postadministration (2, 4, 6, 8, 24 and 48 hours). Captopril decreased arterial blood pressure in the SHR. FRP also decreased arterial blood pressure in these animals, but the effect of this extract was always less accentuated than the effect of Captopril. The most effective dose of FRP to decrease SBP in the SHR was 20 mg/kg. This dose of FRP caused in this strain the maximum decrease in SBP (-26.88 ± 3.05 mm Hg) 4 hours post-intragastric administration. Nevertheless, the same dose of the extract did not modify SBP in Wistar-Kyoto rats, that are considered as the normotensive control for the SHR. FRP decreased also DBP in the SHR. The maximum decrease in this variable (-39.16 ± 12.1 mm Hg) was observed 4 hours postadministration of 30 mg/kg FRP. Surprisingly, higher doses of FRP decreased less SBP and DBP in the SHR...
Description
Tesis inédita de la Universidad Complutense de Madrid, Facultad de Medicina, Departamento de Farmacología, leída el 13-03-2014