Universidad Complutense de Madrid
E-Prints Complutense

Structure-activity relationship of α mating pheromone from the fungal pathogen Fusarium oxysporum

Impacto

Downloads

Downloads per month over past year



Vitale, Stefania and Partida Hanon, Angelica and Serrano, Soraya and Martínez del Pozo, Álvaro and Di Pietro, Antonio and Turrà, David and Bruix, Marta (2017) Structure-activity relationship of α mating pheromone from the fungal pathogen Fusarium oxysporum. Journal of Biological Chemistry . ISSN 1083-351X

[img]
Preview
PDF
1MB

Official URL: http://www.jbc.org/content/early/2017/01/18/jbc.M116.766311



Abstract

During sexual development, ascomycete fungi produce two types of peptide pheromones termed a and α. The α pheromone from the budding yeast Saccharomyces cerevisiae, a thirteen residue peptide which elicits cell cycle arrest and chemotropic growth, has served as paradigm for the interaction of small peptides with their cognate G protein-coupled receptors (GPCRs). However, no structural information is currently available for α pheromones from filamentous ascomycetes, which are significantly shorter and share almost no sequence similarity with the S. cerevisiae homolog. High-resolution structure of synthetic α-pheromone from the plant pathogenic ascomycete Fusarium oxysporum revealed the presence of a central β-turn resembling that of its yeast counterpart. Disruption of the fold by Dalanine substitution of the conserved central Gly6-Gln7 residues or by random sequence scrambling demonstrated a crucial role for this structural determinant in chemoattractant activity. Unexpectedly, the growth inhibitory effect of F. oxysporum α-pheromone was independent of the cognate GPCR Ste2 and of the central β-turn but instead required two conserved Trp1-Cys2 residues at the N-terminus. These results indicate that, in spite of their reduced size, fungal α-pheromones contain discrete functional regions with a defined secondary structure that regulate diverse biological processes such as polarity reorientation and cell division.


Item Type:Article
Uncontrolled Keywords:Fusarium oxysporum; α-pheromone; chemotropism; hyphal growth inhibition
Subjects:Sciences > Chemistry > Molecular biology
Sciences > Chemistry > Biochemistry
ID Code:41589
Deposited On:01 Mar 2017 14:09
Last Modified:01 Feb 2018 00:01

Origin of downloads

Repository Staff Only: item control page