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Elevated intraocular pressure increases melatonin levels in the aqueous humour

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Alkozi, Hanan Awad and Sánchez Naves, Juan and Pérez de Lara, María Jesús and Carracedo Rodríguez, Juan Gonzalo and Fonseca, Begoña and Martínez Águila, Alejandro and Pintor, Jesús (2016) Elevated intraocular pressure increases melatonin levels in the aqueous humour. Acta Ophthalmologica . pp. 1-5. ISSN 1755-375X

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Official URL: http://dx.doi.org/10.1111/aos.13253



Abstract

Purpose: To study the levels of melatonin in the aqueous humour of normotensive and hypertensive intraocular pressure (IOP) patients and to compare them to an animal model of glaucoma.
Methods : A total of 37 eyes of 37 patients who underwent cataract surgery were included in the study and were divided into normotensive patients, with IOP below 21 mmHg (n = 23), and hypertensive patients, with IOP > 21 mmHg (n = 14). Glaucomatous DBA/2J (n = 6) and control C57BL/6J (n = 6) mice presenting 3 and 12 months of age for each strain were also used. Human and mice aqueous humours were aspirated using a 30-gauge Rycroft cannula on a tuberculin syringe and further processed to quantify melatonin by high-performance liquid chromatography analysis.
Results: Melatonin levels in normotensive patients (IOP below 21 mmHg) presented values as medians (first quartile; third quartile) of 14.62 (5.38;37.99) ng/ml (n = 23), while hypertensive patients (IOP above 21 mmHg) showed melatonin concentrations of 46.63 (10.28; 167.28) ng/ml (n = 14; p < 0.039). Glaucoma mice presented melatonin values of 0.37 (0.34; 0.59) ng/ml (at 3 months of age, before the pathology starts), which increased to 1.55 (0.94; 1.88) ng/ml (at 12 months of age, when the pathology is fully developed and IOP is maximum; n = 6, p < 0.001). Control mice did not significantly modified melatonin concentrations between 3 and 12 months of age.
Conclusion: Patients with high IOP present increased concentrations of melatonin in their aqueous humour compared to normotensive patients. This has been confirmed in a glaucomatous animal model in which it has been possible to see a correlation between the development of the pathology, with an increase in IOP, and a concomitant elevation of melatonin in the aqueous humour.


Item Type:Article
Additional Information:

Received on May 2nd, 2016.
Accepted on July 31st, 2016.

Uncontrolled Keywords:Aqueous humour ; Glaucoma ; High-performance liquid chromatography ; Humans ; Melatonin ; Mice
Subjects:Medical sciences > Medicine > Ophtalmology
Medical sciences > Optics > Physiological optics
ID Code:41664
Deposited On:13 Mar 2017 08:22
Last Modified:13 Mar 2017 08:22

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