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Antigenicity of Anisakis simplex s.s. L3 in parasitized fish after heating conditions used in the canning processing



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Tejada, Margarita y Olivares, Fabiola y de las Heras, Cristina y Careche, Mercedes y Solas Alados, Mª Teresa y García, María Luisa y Fernández, Agustín y Mendizábal, Ángel y Navas, Alfonso y Rodríguez Mahillo, Ana Isabel y González Muñoz, Miguel (2015) Antigenicity of Anisakis simplex s.s. L3 in parasitized fish after heating conditions used in the canning processing. Journal of the Science of Food and Agriculture, 95 . pp. 922-927. ISSN 0022-5142

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URL Oficial: http://onlinelibrary.wiley.com/journal/10.1002/(ISSN)1097-0010


BACKGROUND: Some technological and food processing treatments applied to parasitized fish kill the Anisakis larvae andprevent infection and sensitization of consumers. However, residual allergenic activity of parasite allergens has been shown.The aim here was to study the effec t of different heat treatments used in the fish canning processing industry on the antigenrecognition of Anisakis L3.Bigeyetuna(Thunnus obesus) and yellowfin tuna (Thunnus albacares) were experimentally infectedwith live L3 Anisakis.After48hat5± 1∘C, brine was added to the muscle, which was then canned raw (live larvae) or heated(90∘C, 30 min) (dead larvae) and treated at 113∘C for 60 min or at 115∘C for 90 min. Anisakis antigens and Ani s 4 were detectedwith anti-crude extract and anti-Ani s 4 antisera respectively.
RESULTS: Ani s 4 decreased in all lots, but the muscle retained part of the allergenicity irrespective of the canning method,as observed by immunohistochemistry. Dot blot analysis showed a high loss of Ani s 4 recognition after canning, but residualantigenicity was present.
CONCLUSION: The results indicate that heat treatment for sterilization under the conditions studied produces a decrease inAni s 4 and suggest a p otential exposure risk for Anisakis-sensitized patients.

Tipo de documento:Artículo
Palabras clave:Anisakis simplex; canning; allergens; Ani s 4; immunohistochemistry; dot blot; Antigens
Materias:Ciencias Biomédicas > Biología
Ciencias Biomédicas > Biología > Microbiología
Ciencias Biomédicas > Biología > Peces
Código ID:41942
Depositado:27 Mar 2017 17:43
Última Modificación:28 Mar 2017 08:23

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