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Cisplatin-induced hearing loss does not correlate with intracellular platinum concentration

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Ramírez Camacho, R. and Esteban Fernández, Diego and Verdaguer, J.M. and Gómez Gómez, M. Milagros and Trinidad, A. and García Berrocal, J.R. and Palacios Corvillo, M. Antonia (2008) Cisplatin-induced hearing loss does not correlate with intracellular platinum concentration. Acta Oto-Laryngologica, 128 (5). pp. 505-509. ISSN 0001-6489 (Print) 1651-2251 (Online)

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Official URL: http://www.tandfonline.com/doi/full/10.1080/00016480701635167



Abstract

Conclusion. Inductively coupled plasma mass spectrometry (ICP-MS) can be applied to organic tissues obtained from experimental animals. Hearing loss does not correlate with the platinum (Pt) concentration found in the inner ear. Drug structure and affinity to inner ear proteins could explain ototoxicity caused by cisplatin. Objectives. To analyse Pt affinity for brain and ear tissues (of similar embryologic origin) in the Wistar rat and clearance gradient after a single dose, and to correlate these findings with hearing changes. Materials and methods. Thirty-two Wistar rats were intraperitoneally injected with cisplatin at a dose of 5 mg/kg. Animals were sacrificed after obtaining auditory brain responses (ABRs) at 3, 7, 30 and 90 days (nine, seven, seven and nine animals, respectively). Brain and both temporal bones were extracted from each animal and analysed by ICP-MS to determine the absolute concentrations of the metal. Eight non-treated animals were employed as a control group. Results. The ABR thresholds were significantly elevated in animals from all groups after cisplatin treatment. A maximum accumulation of Pt for inner ear and brain was revealed around the first week: 3.175 (57%) and 0.342 (72%), respectively. Pt significantly accumulated in greater quantities in ear than in brain (pB0.01) and was cleared at a higher rate in brain than in ear (pB0.01) following cochlea/brain ratio analysis. No statistically significant correlation was found between amounts of Pt and hearing loss in the study animals.


Item Type:Article
Uncontrolled Keywords:Hair cell, total Pt, ICP-MS, cisplatin ototoxicity, Wistar rat
Subjects:Sciences > Chemistry > Analytic chemistry
ID Code:42144
Deposited On:06 Apr 2017 11:18
Last Modified:07 Apr 2017 07:47

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