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Species-specific optical genosensors for the detection of mycotoxigenic Fusarium fungi in food samples

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Peltomaa, Riikka and Vaghini, Silvia and Patiño Álvarez, Belén and Benito-Peña, Elena and Moreno-Bondi, María C. (2016) Species-specific optical genosensors for the detection of mycotoxigenic Fusarium fungi in food samples. Analytica Chimica Acta, 935 . pp. 231-238. ISSN 0003-2670, ESSN: 1873-4324

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Official URL: http://www.sciencedirect.com/science/article/pii/S0003267016307711



Abstract

Plant-pathogenic Fusarium species, Fusarium verticillioides and Fusarium proliferatum, are the major producers of fumonisins which are one of the most common mycotoxins found in maize. Herein, we report the development of specific and sensitive genosensors for detecting these two closely related Fusarium species in food samples. The sensors are based on species-specific capture and detection probes, which bind to the intergenic spacer region of rDNA (IGS). Oligonucleotide functionalized magnetic microbeads are used to capture the target DNA which is then detected using biotinylated detection probes and a streptavidin-coupled label. The developed genosensors had detection limits of 1.8 pM and 3.0 pM for F. proliferatum and F. verticillioides, respectively, using synthetic DNA targets. Furthermore, the biosensors were used to analyze natural fungal contamination of commercial maize samples. After amplification of the genomic DNA the sensors detected the presence of the fungi, in accordance with previous results obtained with PCR. No cross-reactivity between F. verticillioides and F. proliferatum, or other fungi species tested, was observed. The developed biosensors can provide a valuable tool to evaluate the potential for mycotoxin contamination in conditions where detection of mycotoxins directly is challenging.


Item Type:Article
Uncontrolled Keywords:Fusarium; Magnetic microbeads; Hybridization assay; Intergenic spacer region
Subjects:Medical sciences > Biology > Microbiology
ID Code:42292
Deposited On:24 Apr 2017 15:09
Last Modified:10 Dec 2018 15:30

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