Universidad Complutense de Madrid
E-Prints Complutense

Blockage of neonatal leptin signaling induces changes in the hypothalamus associated with delayed pubertal onset and modifications in neuropeptide expression during adulthood in male rats

Impacto

Downloads

Downloads per month over past year

Mela Rivas, Virginia and Jiménez, Sara and Freire Regatillo, Alejandra and Barrios, Vicente and Marco López, Eva and López Rodríguez, Ana Belén and Argente, Jesús and Viveros, María Paz and Chowen, Julie A. (2016) Blockage of neonatal leptin signaling induces changes in the hypothalamus associated with delayed pubertal onset and modifications in neuropeptide expression during adulthood in male rats. Peptides, 86 . pp. 63-71. ISSN 0196-9781

[img] PDF
Restringido a Repository staff only

1MB

Official URL: https://www.journals.elsevier.com/peptides



Abstract

The neonatal leptin surge, occurring from postnatal day (PND) 5 to 13 and peaking at PND9 in rodents, is important for the development of neuroendocrine circuits involved in metabolic control and reproductive function. We previously demonstrated that treatment with a leptin antagonist from PND 5 to 9, coincident with peak leptin levels in the neonatal surge, modified trophic factors and markers of cell turnover and neuronal maturation in the hypothalamus of peri-pubertal rats. The kisspeptin system and metabolic neuropeptide and hormone levels were also modified. Here our aim was to investigate if the timing of pubertal onset is altered by neonatal leptin antagonism and if the previously observed peripubertal modifications in hormones and neuropeptides persist into adulthood and affect male sexual behavior. To this end, male Wistar rats were treated with a pegylated super leptin antagonist (5 mg/kg, s.c.) from PND 5 to 9 and killed at PND102–103. The appearance of external signs of pubertal onset was delayed. Hypothalamic kiss1 mRNA levels were decreased in adult animals, but sexual behavior was not significantly modified. Although there was no effect on body weight or food intake, circulating leptin, insulin and triglyceride levels were increased, while hypothalamic leptin receptor, POMC and AgRP mRNA levels were decreased. In conclusion, alteration of the neonatal leptin surge can modify the timing of pubertal onset and have long-term effects on hypothalamic expression of reproductive and metabolic neuropeptides.


Item Type:Article
Uncontrolled Keywords:Leptin antagonist; Kisspeptin; Puberty; Sexual behavior
Subjects:Medical sciences > Biology
Medical sciences > Biology > Animal physiology
ID Code:42424
Deposited On:27 Apr 2017 16:29
Last Modified:09 May 2017 10:26

Origin of downloads

Repository Staff Only: item control page