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Analysis of gene expression of bifidobacteria using as the reporter an anaerobic fluorescent protein

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Montenegro-Rodríguez, Cynthia and Peirotén, Angela and Sánchez Jiménez, Abel and Arqués, Juan Luis and Landete, José María (2015) Analysis of gene expression of bifidobacteria using as the reporter an anaerobic fluorescent protein. Biotechnology Letters, 37 (1). pp. 1405-1413. ISSN 0141-5492, ESSN: 1573-6776

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Official URL: https://link.springer.com/article/10.1007/s10529-015-1802-8



Abstract

To determine the effectiveness of evoglow-Pp1 as a reporter to study gene expression in bifidobacteria. To choose a strong and constitutive promoter to track fluorescently labelled bifidobacteria in environments under anaerobic conditions. Results The elongation factor P (EF-P) promoter from Bifidobacterium longum CECT 4551 produced the highest emission of fluorescence signal and was therefore able to produce the highest gene expression of the promoters studied. The promoters from B. longum CECT 4551 showed different fluorescence signal intensities which, in descending order, were: EF-P, initiation factor IF-2, elongation factor G, elongation factor Tu, elongation factor Nus A, elongation factor Ts and 30S ribosomal protein S12. Conclusions The consistency of the methods employed (fluorescence imaging system, fluorescence microscopy, fluorimetry and flow cytometry) showed that the construction pNZ:Prom.GFPana contained the anaerobic fluorescent protein evoglow-Pp1 could be exploited as a tool for analysing the gene expression in bifidobacteria strains.


Item Type:Article
Uncontrolled Keywords:Anaerobic fluorescent protein Bifidobacteria Elongation factor P promoter Gene expression
Subjects:Medical sciences > Biology > Microbiology
ID Code:42431
Deposited On:28 Apr 2017 08:39
Last Modified:10 Dec 2018 15:25

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