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Topographical and mechanical characterization of living eukaryotic cells on opaque substrates: development of a general procedure and its application to the study of non-adherent lymphocytes

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Daza, Rafael and Cruces González, Julia and Arroyo Hernández, María and Marí-Buyé, Núria and Fuente del Rey, Mónica de la and Plaza, Gustavo R. and Elices, Manuel and Pérez Rigueiro, José and Guinea, Gustavo V. (2015) Topographical and mechanical characterization of living eukaryotic cells on opaque substrates: development of a general procedure and its application to the study of non-adherent lymphocytes. Physical Biology, 12 (2). pp. 1-12. ISSN 1478-3967, ESSN: 1478-3975

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Official URL: http://iopscience.iop.org/article/10.1088/1478-3975/12/2/026005/meta



Abstract

The mechanical behavior of living murine T-lymphocytes was assessed by atomic force microscopy (AFM). A robust experimental procedure was developed to overcome some features of lymphocytes, in particular their spherical shape and non-adherent character. The procedure included the immobilization of the lymphocytes on amine-functionalized substrates, the use of hydrodynamic effects on the deflection of the AFM cantilever to monitor the approaching, and the use of the jumping mode for obtaining the images. Indentation curves were analyzed according to Hertz's model for contact mechanics. The calculated values of the elastic modulus are consistent both when considering the results obtained from a single lymphocyte and when comparing the curves recorded from cells of different specimens.


Item Type:Article
Uncontrolled Keywords:Atomic force microscopy; Cell; Mechanics
Subjects:Medical sciences > Medicine > Immunology
Medical sciences > Biology > Cytology
ID Code:42521
Deposited On:05 May 2017 10:40
Last Modified:05 May 2017 11:43

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