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Anatomical distribution and daily profile of gper1b gene expression in brain and peripheral structures of goldfish (Carassius auratus)



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Sánchez Bretaño, Aída y Gueguen, Marie-M. y Cano-Nicolau, Joel y Kah, Olivier y Alonso Gómez, Ángel Luis y Delgado Saavedra, María Jesús y Isorna Alonso, Esther (2015) Anatomical distribution and daily profile of gper1b gene expression in brain and peripheral structures of goldfish (Carassius auratus). Chronobiology International, 32 (7). pp. 889-902. ISSN 0742-0528, ESSN: 1525-6073

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URL Oficial: http://www.tandfonline.com/doi/full/10.3109/07420528.2015.1049615


The functional organization of the circadian system and the location of the main circadian oscillators vary through phylogeny. Present study investigates by in situ hybridization the anatomical location of the clock gene gPer1b in forebrain and midbrain, pituitary, and in two peripheral locations, the anterior intestine and liver, in a teleost fish, the goldfish (Carassius auratus). Moreover, the daily expression profiles of this gene were also studied by quantitative Real Time-PCR. Goldfish were maintained under a 12L–12D photoperiod and fed daily at 2 h after lights were switched on. A wide distribution of gPer1b mRNA in goldfish brain and pituitary was found in telencephalon, some hypothalamic nuclei (including the homologous to mammalian SCN), habenular nucleus, optic tectum, cerebellum and torus longitudinalis. Moreover, gPer1b expression was observed, for the first time in teleosts, in the pituitary, liver and anterior intestine. Day/night differences in gper1b mRNA abundance were found by in situ hybridization, with higher signal at nighttime that correlates with the results obtained by RT-PCR, where a rhythmic gPer1b expression was found in all tissues with acrophases at the end of the night. Amplitudes of gper1b rhythms vary among tissues, being higher in liver and intestine than in the brain, maybe because different cues entrain clocks in these locations. These results support the existence of functional clocks in many central and peripheral locations in goldfish coordinated, ticking at the same time.

Tipo de documento:Artículo
Palabras clave:Brain; Circadian system; Clock gene; In situ hybridization; Intestine; Liver; Pituitary
Materias:Ciencias Biomédicas > Biología > Fisiología animal
Ciencias Biomédicas > Biología > Peces
Código ID:42587
Depositado:08 May 2017 11:00
Última Modificación:08 May 2017 14:06

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