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CB2 cannabinoid receptor is involved in the anti-inflammatory effects of leptin in a model of traumatic brain injury

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López Rodríguez, Ana Belén and Mela Rivas, Virginia and Acaz Fonseca, Estefanía and García Segura, Luis Miguel and Viveros, María Paz (2016) CB2 cannabinoid receptor is involved in the anti-inflammatory effects of leptin in a model of traumatic brain injury. Experimental Neurology, 279 . pp. 274-282. ISSN 0014-4886

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Abstract

Background and purpose
The rates for traumatic brain injury (TBI) have risen in the last decade. Studies in animal models and clinical trials have not yet resulted in an effective treatment for TBI. Leptin, a 16 kDa peptidic hormone is mainly known as a regulator of energy balance and has been shown to exert neuroprotective effects in different models of brain pathology. In this study, we have assessed whether leptin exerts protective actions in a TBI mouse model. In addition, the possible implication of CB2 cannabinoid receptor in leptin actions has been explored, since it is known that the endocannabinoid system interacts with leptin and actively participates in brain recovery after lesions.
Methods
Swiss (CD1) male mice were subjected to weigh-drop model for TBI. Prior to the lesion, mice were injected with an antagonist of CB2 receptor (AM630) or the vehicle and immediately after TBI, they received leptin or vehicle treatment. Data were analyzed using a two-way ANOVA or the non-parametric test Kruskal–Wallis when appropriate. For correlation analyses, Spearman's rho test, followed by linear regression test, was used.
Results
TBI induced a neurological deficit, which was improved by leptin treatment. Leptin recovered several parameters affected by TBI, including the expression of cannabinoid receptors, axonal injury marker and neuroinflammatory components. The effects of leptin were prevented or reduced when it was administered in combination with the CB2 receptor antagonist, AM630.
Conclusions and implications
Since some of the beneficial effects of leptin were not evident in the presence of AM630, our results suggest that CB2 receptor might be involved in the full expression of the neuroprotective effects of the hormone. These findings open new avenues for the study of leptin as a therapeutic treatment for TBI and enhance the importance of CB2 receptor in TBI pathophysiology and recovery.


Item Type:Article
Uncontrolled Keywords:Traumatic brain injury; CB2 cannabinoid receptor antagonist; Leptin; Neuroinflammation; Mice
Subjects:Medical sciences > Biology
Medical sciences > Biology > Animal physiology
ID Code:42600
Deposited On:08 May 2017 13:11
Last Modified:08 May 2017 13:55

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