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TRPV4 Stimulation Induced Melatonin Secretion by Increasing Arylalkymine N-acetyltransferase (AANAT) Protein Level

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Alkozi, Hanan Awad and Pérez de Lara, María Jesús and Sánchez Naves, Juan and Pintor, Jesús (2017) TRPV4 Stimulation Induced Melatonin Secretion by Increasing Arylalkymine N-acetyltransferase (AANAT) Protein Level. International Journal of Molecular Sciences, 18 (4). p. 746. ISSN 1422-0067

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Official URL: http://dx.doi.org/10.3390/ijms18040746




Abstract

Melatonin is a molecule which has gained a great deal of interest in many areas of science; its synthesis was classically known to be in the pineal gland. However, many organs synthesize melatonin, such as several ocular structures. Melatonin is known to participate in many functions apart from its main action regulating the circadian rhythm. It is synthesized from serotonin in two steps, with a rate-limiting step carried out by arylalkymine N-acetyltransferase (AANAT). In this report, the role of TRPV4 channel present in human ciliary body epithelial cells in AANAT production was studied. Several experiments were undertaken to verify the adequate time to reach the maximal effect by using the TRPV4 agonist GSK1016790A, together with a dose–response study. An increase of 2.4 folds in AANAT was seen after 18 h of incubation with 10 nM of GSK1016790A (p < 0.001, n = 6). This increment was verified by antagonist assays. In summary, AANAT levels and therefore melatonin synthesis change after TRPV4 channel stimulation. Using this cell model together with human ciliary body tissue it is possible to suggest that AANAT plays an important role in pathologies related to intraocular pressure.


Item Type:Article
Additional Information:

Received: 5 March 2017 / Revised: 21 March 2017 / Accepted: 27 March 2017 / Published: 1 April 2017
(This article belongs to the Special Issue Melatonin and Its Analogues: Experimental and Clinical Aspects)

Uncontrolled Keywords:AANAT; Ciliary body; Eye; Melatonin; TRPV4
Subjects:Medical sciences > Medicine > Biochemistry
Medical sciences > Medicine > Ophtalmology
ID Code:42824
Deposited On:29 May 2017 08:53
Last Modified:06 Jun 2017 07:44

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