Universidad Complutense de Madrid
E-Prints Complutense

Involvement of the Cohesin Cofactor PDS5 (SPO76) During Meiosis and DNA Repair in Arabidopsis thaliana

Impacto

Downloads

Downloads per month over past year



Pradillo Orellana, Mónica and Knoll, Alexander and Oliver Velasco, Cecilia and Varas, Javier and Corredor Molguero, Eduardo and Puchta, Olger and Santos Coloma, Juan Luis (2015) Involvement of the Cohesin Cofactor PDS5 (SPO76) During Meiosis and DNA Repair in Arabidopsis thaliana. Frontiers in Plant Science, 6 . ISSN 1664-462X

[img]
Preview
PDF
Creative Commons Attribution.

6MB

Official URL: http://journal.frontiersin.org/journal/plant-science



Abstract

Maintenance and precise regulation of sister chromatid cohesion is essential for faithful chromosome segregation during mitosis and meiosis. Cohesin cofactors contribute to cohesin dynamics and interact with cohesin complexes during cell cycle. One of these, PDS5, also known as SPO76, is essential during mitosis and meiosis in several organisms and also plays a role in DNA repair. In yeast, the complex Wapl-Pds5 controls cohesion maintenance and colocalizes with cohesin complexes into chromosomes. In Arabidopsis, AtWAPL proteins are essential during meiosis, however, the role of AtPDS5 remains to be ascertained. Here we have isolated mutants for each of the five AtPDS5 genes (A–E) and obtained, after different crosses between them, double, triple, and even quadruple mutants (Atpds5a Atpds5b Atpds5c Atpds5e). Depletion of AtPDS5 proteins has a weak impact on meiosis, but leads to severe effects on development, fertility, somatic homologous recombination (HR) and DNA repair. Furthermore, this cohesin cofactor could be important for the function of the AtSMC5/AtSMC6 complex. Contrarily to its function in other species, our results suggest that AtPDS5 is dispensable during the meiotic division of Arabidopsis, although it plays an important role in DNA repair by HR.


Item Type:Article
Uncontrolled Keywords:Arabidopsis thaliana, cohesin cofactor, DNA repair, homologous recombination, Meiosis, PDS5, SPO76
Subjects:Medical sciences > Biology > Botany
Medical sciences > Biology > Genetics
ID Code:42850
Deposited On:22 May 2017 14:52
Last Modified:10 Dec 2018 15:30

Origin of downloads

Repository Staff Only: item control page