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D-Root: a system for cultivating plants with the roots indarkness or under different light conditions

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Silva Navas, Javier and Moreno Risueno, Miguel A. and Manzano, Concepción and Pallero Baena, Mercedes and Navarro Neila, Sara and Téllez Robledo, Bárbara and García Mina, José M. and Baigorri, Roberto and Gallego Rodríguez, Francisco Javier and Pozo, Juan C. del (2015) D-Root: a system for cultivating plants with the roots indarkness or under different light conditions. Plant Journal, 84 (1). pp. 244-255. ISSN 0960-7412

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Official URL: http://onlinelibrary.wiley.com/journal/10.1111/(ISSN)1365-313X



Abstract

In nature roots grow in the dark and away from light (negative phototropism). However, most currentresearch in root biology has been carried out with the root system grown in the presence of light. Here, wehave engineered a device, called Dark-Root (D-Root), to grow plants in vitro with the aerial part exposed tothe normal light/dark photoperiod while the roots are in the dark or exposed to specific wavelengths orlight intensities. D-Root provides an efficient system for cultivating a large number of seedlings and easilycharacterizing root architecture in the dark. At the morphological level, root illumination shortens rootlength and promotes early emergence of lateral roots, therefore inducing expansion of the root system. Sur-prisingly, root illumination also affects shoot development, including flowering time. Our analyses alsoshow that root illumination alters the proper response to hormones or abiotic stress (e.g. salt or osmoticstress) and nutrient starvation, enhancing inhibition of root growth. In conclusion, D-Root provides a grow-ing system closer to the natural one for assaying Arabidopsis plants, and therefore its use will contribute toa better understanding of the mechanisms involved in root development, hormonal signaling and stressresponses.


Item Type:Article
Uncontrolled Keywords:root development, light, hormone response, abiotic stress, reactive oxygen species, technicalAdvance.
Subjects:Medical sciences > Biology > Botany
Medical sciences > Biology > Genetics
ID Code:43076
Deposited On:01 Jun 2017 14:32
Last Modified:10 Dec 2018 15:30

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