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Proteomic and Lipidomic Analysis of Nanoparticle Corona upon Contact with Lung Surfactant Reveals Differences in Protein, but Not Lipid Composition

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Raesch, Simon Sebastian and Tenzer, Stefan and Storck, Wiebke and Rurainski, Alexander and Selzer, Dominik and Ruge, Christian Arnold and Pérez Gil, Jesús and Schaefer, Ulrich Friedrich and Lehr, Claus Michael (2015) Proteomic and Lipidomic Analysis of Nanoparticle Corona upon Contact with Lung Surfactant Reveals Differences in Protein, but Not Lipid Composition. ACS Nano, 9 (12). pp. 11872-11885. ISSN 1936-0851

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Official URL: http://pubs.acs.org/journal/ancac3



Abstract

Pulmonary surfactant (PS) constitutes the first line of host defense in the deep lung. Because of its high content of phospholipids and surfactant specific proteins, the interaction of inhaled nanoparticles (NPs) with the pulmonary surfactant layer is likely to form a corona that is different to the one formed in plasma. Here we present a detailed lipidomic and proteomic analysis of NP corona formation using native porcine surfactant as a model. We analyzed the adsorbed biomolecules in the corona of three NP with different surface properties (PEG-, PLGA-, and Lipid-NP) after incubation with native porcine surfactant. Using label-free shotgun analysis for protein and LC MS for lipid analysis, we quantitatively determined the corona composition. Our results show a conserved lipid composition in the coronas of all investigated NPs regardless of their surface properties, with only hydrophilic PEG-NPs adsorbing fewer lipids in total. In contrast, the analyzed NP displayed a marked difference in the protein corona, consisting of up to 417 different proteins. Among the proteins showing significant differences between the NP coronas, there was a striking prevalence of molecules with a notoriously high lipid and surface binding, such as, e.g., SP-A, SP-D, DMBT1. Our data indicate that the selective adsorption of proteins mediates the relatively similar lipid pattern in the coronas of different NPs. On the basis of our lipidomic and proteomic analysis, we provide a detailed set of quantitative data on the composition of the surfactant corona formed upon NP inhalation, which is unique and markedly different to the plasma corona.


Item Type:Article
Uncontrolled Keywords:bronchoalveolar lining fluid, nanoparticle surface, biological barrier, bionano interface, inhalation, lipid-protein interaction, magnetic separation
Subjects:Medical sciences > Biology
Medical sciences > Biology > Molecular biology
ID Code:43187
Deposited On:07 Jun 2017 17:29
Last Modified:09 Jun 2017 07:52

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