Universidad Complutense de Madrid
E-Prints Complutense

Functional organization of the HIV lipid envelope

Impacto

Downloads

Downloads per month over past year

Huarte, Nerea and Carravilla, Pablo and Cruz Rodríguez, Antonio and Lorizate, Maier and Nieto-Garay, Jon A. and Kräusslich, Hans-Georg and Pérez Gil, Jesús and Requejo Isidro, José and Nieva, José L. (2016) Functional organization of the HIV lipid envelope. Scientific Reports, 6 . pp. 1-14. ISSN 2045-2322, ESSN: 2045-2322

[img] PDF
Creative Commons Attribution.

2MB

Official URL: https://www.nature.com/articles/srep34190



Abstract

The chemical composition of the human immunodeficiency virus type 1 (HIV-1) membrane is critical for fusion and entry into target cells, suggesting that preservation of a functional lipid bilayer organization may be required for efficient infection. HIV-1 acquires its envelope from the host cell plasma membrane at sites enriched in raft-type lipids. Furthermore, infectious particles display aminophospholipids on their surface, indicative of dissipation of the inter-leaflet lipid asymmetry metabolically generated at cellular membranes. By combining two-photon excited Laurdan fluorescence imaging and atomic force microscopy, we have obtained unprecedented insights into the phase state of membranes reconstituted from viral lipids (i.e., extracted from infectious HIV-1 particles), established the role played by the different specimens in the mixtures, and characterized the effects of membrane-active virucidal agents on membrane organization. In determining the molecular basis underlying lipid packing and lateral heterogeneity of the HIV-1 membrane, our results may help develop compounds with antiviral activity acting by perturbing the functional organization of the lipid envelope.


Item Type:Article
Uncontrolled Keywords:Chemical biology; Fluorescence imaging; Membrane structure and assembly; Viral membrane fusion
Subjects:Medical sciences > Biology > Biochemistry
ID Code:43222
Deposited On:13 Jun 2017 11:37
Last Modified:13 Jun 2017 11:54

Origin of downloads

Repository Staff Only: item control page