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Effect of lung surfactant Protein SP-C and SP-C-Promoted membrane fragmentation on cholesterol dynamics



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Roldán López, Nuria and Nyholm, Thomas K. M. and Slotte, J. Peter and Pérez Gil, Jesús and García Álvarez, Begoña (2016) Effect of lung surfactant Protein SP-C and SP-C-Promoted membrane fragmentation on cholesterol dynamics. Biophysical Journal, 111 (8). pp. 1703-1713. ISSN 0006-3495, ESSN: 1542-0086

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Official URL: http://www.cell.com/biophysj/fulltext/S0006-3495(16)30815-3


To allow breathing and prevent alveolar collapse, lung surfactant (LS) develops a complex membranous system at the respiratory surface. LS is defined by a specific protein and lipid composition, including saturated and unsaturated phospholipid species and cholesterol. Surfactant protein C (SP-C) has been suggested to be an essential element for sustaining the presence of cholesterol in surfactant without functional impairment. In this work, we used a fluorescent sterol-partitioning assay to assess the effect of the surfactant proteins SP-B and SP-C on cholesterol distribution in membranes. Our results suggest that in the LS context, the combined action of SP-B and SP-C appears to facilitate cholesterol dynamics, whereas SP-C does not seem to establish a direct interaction with cholesterol that could increase the partition of free cholesterol into membranes. Interestingly, SP-C exhibits a membrane-fragmentation behavior, leading to the conversion of large unilamellar vesicles into highly curved vesicles ~25 nm in diameter. Sterol partition was observed to be sensitive to the bending of bilayers, indicating that the effect of SP-C to mobilize cholesterol could be indirectly associated with SP-C-mediated membrane remodeling. Our results suggest a potential role for SP-C in generating small surfactant structures that may participate in cholesterol mobilization and pulmonary surfactant homeostasis at the alveolar interfaces.

Item Type:Article
Uncontrolled Keywords:Lung surfactan Protein SP-C; Membrane fragmentation; Cholesterol
Subjects:Medical sciences > Biology > Biochemistry
ID Code:43462
Deposited On:21 Jun 2017 08:40
Last Modified:21 Jun 2017 11:04

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