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Development of an immobilized biocatalyst based on Bacillus psychrosaccharolyticus NDT for the preparative synthesis of trifluridine and decytabine

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Fresco-Taboada, Alba and Serra, Immacolata and Arroyo Sánchez, Miguel and Fernández-Lucas, Lucas and Mata Riesco, Isabel de la and Terreni, Marco (2016) Development of an immobilized biocatalyst based on Bacillus psychrosaccharolyticus NDT for the preparative synthesis of trifluridine and decytabine. Catalysis Today, 259 (Part 1). pp. 197-204. ISSN 0920-5861

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Official URL: http://www.sciencedirect.com/science/article/pii/S0920586115004836



Abstract

The immobilization of Bacillus psychrosaccharolyticus nucleoside 2 -deoxyribosyltransferase was deeply investigated and finally optimized. The best immobilization procedure resulted to be ionic adsorption on PEI 600 Da agarose followed by crosslinking with 70% oxidized dextran (20 kDa). The percentage of recovered activity was further improved (from 21% to 33%) by the addition of 20% glycerol to the immobilization mixture. The resulting biocatalyst showed a stability profile similar to that of the soluble enzyme and it was used for the preparative synthesis oftrifluridine and decytabine obtaining conversions ranging from 50% to 76%.


Item Type:Article
Uncontrolled Keywords:Nucleoside 2′-deoxyribosyltransferase; Biotransformations; Enzyme immobilization; Nucleoside analogues; Decytabine; Trifluridine
Subjects:Medical sciences > Biology > Biochemistry
ID Code:43694
Deposited On:30 Jun 2017 11:43
Last Modified:03 Jul 2017 07:32

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