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Pinpointing cryptic borders: Fine-scale phylogeography and genetic landscape analysis of the Hormogaster elisae complex (Oligochaeta,Hormogastridae)

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Fernández Marchán, Daniel and Fernández, Rosa and Sosa, Irene de and Díaz Cosín, Darío J. and Novo Rodríguez, Marta (2017) Pinpointing cryptic borders: Fine-scale phylogeography and genetic landscape analysis of the Hormogaster elisae complex (Oligochaeta,Hormogastridae). Molecular Phylogenetics and Evolution, 112 . pp. 185-193. ISSN 1055-7903, ESSN: 1095-9513

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Official URL: http://www.sciencedirect.com/science/article/pii/S1055790317300805



Abstract

Spatial and temporal aspects of the evolution of cryptic species complexes have received less attention than species delimitation within them. The phylogeography of the cryptic complex Hormogaster elisae (Oligochaeta, Hormogastridae) lacks knowledge on several aspects, including the small-scale distribution of its lineages or the palaeogeographic context of their diversification. To shed light on these topics, a dense specimen collection was performed in the center of the Iberian Peninsula – resulting in 28 new H. elisae collecting points, some of them as close as 760 m from each other- for a higher resolution of the distribution of the cryptic lineages and the relationships between the populations. Seven molecular regions were amplified: mitochondrial subunit 1 of cytochrome c oxidase (COI), 16S rRNA and tRNA Leu, Ala, and Ser (16S t-RNAs), one nuclear ribosomal gene (a fragment of 28S rRNA) and one nuclear protein-encoding gene (histone H3) in order to infer their phylogenetic relationships. Different representation methods of the pairwise divergence in the cytochrome oxidase I sequence (heatmap and genetic landscape graphs) were used to visualize the genetic structure of H. elisae. A nested approach sensu Mairal et al. (2015) (connecting the evolutionary rates of two datasets of different taxonomic coverage) was used to obtain one approximation to a time-calibrated phylogenetic tree based on external Clitellata fossils and a wide molecular dataset. Our results indicate that limited active dispersal ability and ecological or biotic barriers could explain the isolation of the different cryptic lineages, which never co-occur. Rare events of long distance dispersal through hydrochory appear as one of the possible causes of range expansion.


Item Type:Article
Uncontrolled Keywords:Cryptic species; Genetic diversity; Haplotype networks; Nuclear markers; Earthworms
Subjects:Medical sciences > Biology > Invertebrates
ID Code:43983
Deposited On:19 Jul 2017 11:45
Last Modified:10 Dec 2018 15:25

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