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Detection of pistachio allergen coding sequences in food products: A comparison of two real time PCR approaches

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Sanchiz, África and Ballesteros, Isabel and Martín, A. and Rueda Muñoz de San Pedro, Julia and Martín Pedrosa, Mercedes and Diéguez, Carmen and Rovira, Mercè and Cuadrado Vives, María del Carmen and Linacero de la Fuente, Rosario (2017) Detection of pistachio allergen coding sequences in food products: A comparison of two real time PCR approaches. Food Control, 75 . pp. 262-270. ISSN 0956-7135

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Official URL: https://www.journals.elsevier.com/food-control/



Abstract

The labelled of pistachio on food products is mandatory and, as a consequence, the development of suitable analytical methodologies to detect this nut in processed foods is advisable. In this work, two different qPCR assays to detect pistachio, SYBR®Green and locked nucleic acid (LNA) probes, are tested and compared. Pis v allergen coding sequences have been amplified and cloned in different pistachio varieties, and specific primers and probes for each allergen have been designed. According to our results, LNA probe-real time PCR appears to be the most sensitive and specific method, reaching 10 mg/kg of pistachio. The effect of temperature and/or pressure on pistachio DNA detection was also analysed by LNA probe-based qPCR. Data showed a reduced amplificability of pistachio after thermal treatment under pressure, nevertheless, this effect was not observed after boiling. The applicability of this method has been studied by analysing 14 food products and by comparison with a commercial ELISA kit.


Item Type:Article
Uncontrolled Keywords:Pistachio; Allergen detection; Real time PCR; Processed foods
Subjects:Medical sciences > Biology
Medical sciences > Biology > Molecular biology
Medical sciences > Biology > Genetics
ID Code:44000
Deposited On:20 Jul 2017 10:16
Last Modified:10 Dec 2018 15:30

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