Universidad Complutense de Madrid
E-Prints Complutense

The absence of the arabidopsis chaperone complex CAF-1 produces mitotic chromosome abnormalities and changes in the expression profiles of genes involved in DNA repair

Impacto

Downloads

Downloads per month over past year

Varas, Javier and Santos Coloma, Juan Luis and Pradillo Orellana, Mónica (2017) The absence of the arabidopsis chaperone complex CAF-1 produces mitotic chromosome abnormalities and changes in the expression profiles of genes involved in DNA repair. Frontiers in Plant Science, 8 . pp. 1-11. ISSN 1664-462X

[img]
Preview
PDF
Creative Commons Attribution.

6MB

Official URL: http://journal.frontiersin.org/journal/plant-science



Abstract

Chromatin Assembly Factor 1 (CAF-1) is an evolutionary conserved heterotrimeric chaperone complex that facilitates the incorporation of histones H3 and H4 onto newly synthesized DNA. We demonstrate here that the mutant deficient for the large subunit of the complex, fas1-4, and in minor extent, the mutant deficient for the middle subunit, fas2-1, display chromosome abnormalities throughout Arabidopsis mitosis. Among them, we observed multicentromeric chromosomes at metaphase, and chromatid bridges and acentric fragments at anaphase-telophase. 45S rDNA and telomeric sequences were frequently involved in bridges and fragments. Gene expression analysis by real-time qPCR has revealed that several genes related to homologous recombination (HR) and alternative non-homologous end-joining (aNHEJ) are overexpressed in fas1-4. These results concur with previous studies which have indicated that HR may be involved in the progressive loss of 45S rDNA and telomeres displayed by fas mutants. However, increased expression of PARP1, PARP2, and LIG6 in fas1-4, and the phenotype shown by the double mutant fas1 rad51 suggest that aNHEJ should also be responsible for the chromosomal aberrations observed. The activity of different DNA repair pathways in absence of CAF-1 is discussed.


Item Type:Article
Uncontrolled Keywords:Arabidopsis thaliana, DNA double-strand breaks, DNA repair, homologous recombination, non-homologous end-joinin
Subjects:Medical sciences > Biology
Medical sciences > Biology > Genetics
ID Code:44004
Deposited On:20 Jul 2017 11:35
Last Modified:10 Dec 2018 15:30

Origin of downloads

Repository Staff Only: item control page