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Evaluating aflatoxin gene expression in aspergillus section flavi

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Azevedo Rodrigues, Paula Cristina and Gil Serna, Jéssica and González Jaén, María Teresa (2017) Evaluating aflatoxin gene expression in aspergillus section flavi. In Mycotoxigenic Fungi. Methods in Molecular Biology: Methods and Protocols (1542). Springer, pp. 237-247. ISBN 978-1-4939-6705-6 (Print) 978-1-4939-6707-0 (Online)

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Official URL: https://link.springer.com/book/10.1007%2F978-1-4939-6707-0



Abstract

The determination of aflatoxin production ability and differentiation of aflatoxigenic strains can be assessed by monitoring the expression of one or several key genes using reverse transcription polymerase chain reaction (RT-PCR). We herein describe the methods for RNA induction, extraction, and quality determination, and the RT-PCR conditions used to evaluate the ability of a given Aspergillus strain to produce aflatoxins.


Item Type:Book Section
Uncontrolled Keywords:Aspergillus flavus; Mycotoxins; Aflatoxigenic fungi; RNA extraction; RT-PCR; Gel electrophoresis
Subjects:Medical sciences > Biology
Medical sciences > Biology > Genetics
Medical sciences > Biology > Microbiology
ID Code:44036
Deposited On:21 Jul 2017 10:19
Last Modified:10 Dec 2018 15:30

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