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Improved Measurement of Elastic Properties of Cells by Micropipette Aspiration and Its Application to Lymphocytes

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Esteban Manzanares, Gustavo and González Bermúdez, Blanca and Cruces, Julia and Fuente del Rey, Mónica de la and Li, Qingxuan and Guinea, Gustavo V. and Pérez Rigueiro, José and Elices, Manuel and Plaza, Gustavo R. (2017) Improved Measurement of Elastic Properties of Cells by Micropipette Aspiration and Its Application to Lymphocytes. Annals of Biomedical Engineering, 45 . pp. 1375-1385. ISSN 0090-6964

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Official URL: https://link.springer.com/journal/10439



Abstract

Mechanical deformability of cells is an important property for their function and development, as well as a useful marker of cell state. The classical technique of micropipette aspiration allows single-cell studies and we provide here a method to measure the two basic mechanical parameters, elastic modulus and Poisson’s ratio. The proposed method, developed from finite-element analysis of micropipette aspiration experiments, may be implemented in future technologies for the automated measurement of mechanical properties of cells, based on the micropipette aspiration technique or on the cell transit through flow constrictions. We applied this method to measure the elastic parameters of lymphocytes, in which the mechanical properties depend on their activation state. Additionally, we discuss in this work the accuracy of previous models to estimate the elastic modulus of cells, in particular the analytical model by Theret et al., widely used in the field. We show the necessity of using an improved model, taking into account the finite size of the cells, to obtain new insights that may remain hidden otherwise.


Item Type:Article
Uncontrolled Keywords:Cell deformability; Finite-size effect; Poisson’s ratio; Young’s modulus; T cell; Finite-element analysis; Non-linear model; Automatic digital analysis
Subjects:Medical sciences > Biology
Medical sciences > Biology > Animal physiology
ID Code:44315
Deposited On:22 Aug 2017 10:39
Last Modified:22 Aug 2017 11:50

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