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Toward Liquid Biopsy: Determination of the Humoral Immune Response in Cancer Patients Using HaloTag Fusion Protein-Modified Electrochemical Bioplatforms

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Garranzo Asensio, María and Guzmán Aránguez, Ana Isabel and Povés Francés, Carmen and Fernández Aceñero, Mª Jesús and Torrente Rodríguez, Rebeca Magnolia and Ruiz Valdepeñas Montiel, Víctor and Domínguez Muñóz, Gemma and San Frutos Llorente, Luis and Rodríguez Salas, Nuria and Villalba Díaz, Mª Teresa (Mayte) and Pingarrón Carrazon, José Manuel and Campuzano Ruiz, Susana and Barderas Manchado, Rodrigo (2016) Toward Liquid Biopsy: Determination of the Humoral Immune Response in Cancer Patients Using HaloTag Fusion Protein-Modified Electrochemical Bioplatforms. Analytical Chemistry, 88 (24). pp. 12339-12345. ISSN 1520-6882

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Official URL: https://doi.org/10.1021/acs.analchem.6b03526




Abstract

Autoantibodies raised against tumor-associated antigens have shown high promise as clinical biomarkers for reliable diagnosis, prognosis, and therapy monitoring of cancer. An electrochemical disposable biosensor for the specific and sensitive determination of p53-specific autoantibodies has been developed for the first time in this work. This biosensor involves the use of magnetic microcarriers (MBs) modified with covalently immobilized HaloTag fusion p53 protein as solid supports for the selective capture of specific autoantibodies. After magnetic capture of the modified MBs onto screen-printed carbon working electrodes, the amperometric signal using the system hydroquinone/H2O2 was related to the levels of p53-autoantibodies in the sample. The biosensor was applied for the analysis of sera from 24 patients with high-risk of developing colorectal cancer and 6 from patients already diagnosed with colorectal (4) and ovarian (2) cancer. The developed biosensor was able to determine p53 autoantibodies with a sensitivity higher than that of a commercial standard ELISA using a just-in-time produced protein in a simpler protocol with less sample volume and easily miniaturized and cost-effective instrumentation.


Item Type:Article
Additional Information:

Received: September 7, 2016
Accepted: November 16, 2016
Published: November 16, 2016

Uncontrolled Keywords:Inmunology ; Autoantibodies ; Electrochemical biosensors ; Cacer ; Tumors ; Halo Tag Fushion Protein
Subjects:Medical sciences > Medicine > Biochemistry
Medical sciences > Medicine > Immunology
Medical sciences > Medicine > Oncology
Medical sciences > Biology > Molecular biology
ID Code:44389
Deposited On:08 Sep 2017 12:36
Last Modified:12 Sep 2017 11:24

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