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Developing an efficient strategy for immobilization of PHB depolymerase on magnetite-based nanoparticles for degrading polyhydroxybutyrate in acidic conditions

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García Hidalgo, Javier and Acebal Sarabia, Carmen and Mata Riesco, Isabel de la and Arroyo Sánchez, Miguel (2017) Developing an efficient strategy for immobilization of PHB depolymerase on magnetite-based nanoparticles for degrading polyhydroxybutyrate in acidic conditions. Biocatalysis and Biotransformation . pp. 1-10. ISSN 1024-2422, ESSN: 1029-2446

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Official URL: http://www.tandfonline.com/doi/full/10.1080/10242422.2017.1315942



Abstract

Several approaches towards the efficient immobilization of poly-3-hydroxybutyrate (PHB) depolymerase from Streptomyces ascomycinicus (PhaZSa) on magnetic-silanized nanoparticles have been explored in order to accomplish the preparation of an active immobilized biocatalyst in PHB hydrolysis in acid conditions. A simple non-covalent adsorption of PhaZSa to silanized magnetic particles at acidic pH during a short incubation time allowed the preparation of immobilized biocatalysts with increased specific activity. In contrast, covalent binding of PhaZSa to silanized magnetite by different methods led to immobilized biocatalysts whose retained activity were low or void.


Item Type:Article
Uncontrolled Keywords:Streptomyces ascomycinicus; Polyhydroxybutyrate PHB depolymerase; Immobilization
Subjects:Medical sciences > Biology > Biochemistry
ID Code:44434
Deposited On:01 Sep 2017 08:16
Last Modified:04 Sep 2017 11:37

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