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Electrochemical sensor for rapid determination of fibroblast growth factor receptor 4 in raw cancer cell lysates

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Khodarahmi, Reza and Torrente Rodríguez, Rebeca M. and Ruiz-Valdepeñas Montiel, Víctor and Campuzano, Susana and Pedrero Muñoz, María and Farchado, Meryem and Vargas, Eva and Manuel de Villena, F. Javier and Garranzo Asensio, María and Barderas, Rodrigo and Pingarrón Carrazón, José Manuel (2017) Electrochemical sensor for rapid determination of fibroblast growth factor receptor 4 in raw cancer cell lysates. PLOS ONE, 12 (4). ISSN 1932-6203

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Official URL: https://doi.org/10.1371/journal.pone.0175056



Abstract

The first electrochemical immunosensor for the determination of fibroblast growth factor receptor 4 (FGFR4) biomarker is reported in this work. The biosensor involves a sandwich configuration with covalent immobilization of a specific capture antibody onto activated carboxylic-modified magnetic microcarriers (HOOC-MBs) and amperometric detection at disposable carbon screen-printed electrodes (SPCEs). The biosensor exhibits a great analytical performance regarding selectivity for the target protein and a low LOD of 48.2 pg mL-1. The electrochemical platform was successfully applied for the determination of FGFR4 in different cancer cell lysates without any apparent matrix effect after a simple sample dilution and using only 2.5 μg of the raw lysate. Comparison of the results with those provided by a commercial ELISA kit shows competitive advantages by using the developed immunosensor in terms of simplicity, analysis time, and portability and cost-affordability of the required instrumentation for the accurate determination of FGFR4 in cell lysates.


Item Type:Article
Subjects:Sciences > Chemistry > Analytic chemistry
ID Code:44543
Deposited On:25 Sep 2017 10:34
Last Modified:05 Aug 2019 10:19

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