Publication: Electrochemical sensor for rapid determination of fibroblast growth factor receptor 4 in raw cancer cell lysates
Loading...
Official URL
Full text at PDC
Publication Date
2017
Authors
Khodarahmi, Reza
Torrente Rodríguez, Rebeca M.
Ruiz-Valdepeñas Montiel, Víctor
Campuzano, Susana
Farchado, Meryem
Vargas, Eva
Manuel de Villena, F. Javier
Garranzo Asensio, María
Barderas, Rodrigo
Advisors (or tutors)
Editors
Journal Title
Journal ISSN
Volume Title
Publisher
San Francisco, CA : Public Library of Science
Citations
Exportar
Abstract
The first electrochemical immunosensor for the determination of fibroblast growth factor receptor 4 (FGFR4) biomarker is reported in this work. The biosensor involves a sandwich configuration with covalent immobilization of a specific capture antibody onto activated carboxylic-modified magnetic microcarriers (HOOC-MBs) and amperometric detection at disposable carbon screen-printed electrodes (SPCEs). The biosensor exhibits a great analytical performance regarding selectivity for the target protein and a low LOD of 48.2 pg mL-1. The electrochemical platform was successfully applied for the determination of FGFR4 in different cancer cell lysates without any apparent matrix effect after a simple sample dilution and using only 2.5 μg of the raw lysate. Comparison of the results with those provided by a commercial ELISA kit shows competitive advantages by using the developed immunosensor in terms of simplicity, analysis time, and portability and cost-affordability of the required instrumentation for the accurate determination of FGFR4 in cell lysates.