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Bridging the gap between molecular and elemental mass spectrometry: Higher energy collisional dissociation (HCD) revealing elemental information

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Esteban Fernández, Diego and El-Khatib, Ahmed H. and Moraleja San José, Irene and Gómez Gómez, M.Milagros and Linscheid, Michael W. (2014) Bridging the gap between molecular and elemental mass spectrometry: Higher energy collisional dissociation (HCD) revealing elemental information. Analytical Chemistry, 87 (3). pp. 1613-1621. ISSN 0003-2700

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Official URL: http://pubs.acs.org/doi/abs/10.1021/ac5032447



Abstract

Molecular mass spectrometry has been applied to simultaneously obtain molecular and elemental information from metal-containing species. Energy tuning of the higher-energy collision dissociation (HCD) fragmentation cell allows the controlled production of typical peptide fragments or elemental reporter ions informing about the metallic content of the analyzed species. Different instrumental configurations and fragmentation techniques have been tested, and the efficiency extracting the elemental information has been compared. HCD fragmentation operating at very high energy led to the best results. Platinum, lanthanides, and iodine reporter ions from peptides interacting with cisplatin, peptides labeled with lanthanides-MeCAT-IA, and iodinated peptides, respectively, were obtained. The possibility to produce abundant molecular and elemental ions in the same analysis simplifies the correlation between both signals and open pathways in metallomics studies enabling the specific tracking of metal-containing species. The proposed approach has been successfully applied to in solution standards and complex samples. Moreover, interesting preliminary MALDI-imaging experiments have been performed showing similar metal distribution compared to laser ablation (LA)-ICPMS.


Item Type:Article
Subjects:Sciences > Chemistry > Analytic chemistry
ID Code:44628
Deposited On:14 Sep 2017 11:18
Last Modified:14 Sep 2017 11:48

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