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Analysis of intermediates of steroid transformations in resting cells by thin-layer chromatography (TLC)

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Guevara, Govinda and Perera González, Jesús and Navarro Llorens, Juana-María (2017) Analysis of intermediates of steroid transformations in resting cells by thin-layer chromatography (TLC). In Microbial steroids : methods and protocols. Methods in molecular biology (1645). Springer, New York, pp. 347-360. ISBN 978-1-4939-7182-4 (Print) 978-1-4939-7183-1 (Online)

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Official URL: https://link.springer.com/protocol/10.1007/978-1-4939-7183-1_24



Abstract

Thin-layer chromatography (TLC) is a useful and convenient method for the analysis of steroids due to: its simple sample preparation, low time-consuming process, high sensitivity, low equipment investment and capacity to work on many samples simultaneously. Here we describe a TLC easy protocol very useful to analyze steroid molecules derived from a biotransformation carried out in wild-type and mutant resting cells of Rhodococcus ruber strain Chol-4. Following this protocol, we were able to detect the presence or the absence of some well-known intermediates of cholesterol catabolism in Rhodococcus, namely AD, ADD, and 9OHAD.


Item Type:Book Section
Uncontrolled Keywords:Resting cells; Detection; Thin-layer chromatography; TLC, Steroids, AD; ADD; 9OHAD
Subjects:Medical sciences > Biology > Biochemistry
ID Code:44684
Deposited On:22 Sep 2017 11:05
Last Modified:25 Sep 2017 11:15

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