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Electrochemical Magnetic Immunosensors for the Determination of Ceruloplasmin

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Ojeda Fernández, Irene and Moreno Guzmán, María and González Cortés, Araceli and Yáñez Sedeño, Paloma and Pingarrón Carrazón, José Manuel (2013) Electrochemical Magnetic Immunosensors for the Determination of Ceruloplasmin. Electroanalysis, 25 (9). pp. 2166-2174. ISSN (Online )1521-4109

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Official URL: http://onlinelibrary.wiley.com/doi/10.1002/elan.201300269/full



Abstract

Electrochemical immunosensors for ceruloplasmin (Cp) are reported for the first time. Two configurations involving magnetic beads (MBs) functionalized with Protein A or Streptavidin for immobilization of Cp antibodies were compared, using competitive immunoassay with synthesized alkaline phosphatase-Cp conjugate. Upon capturing MBs-immunoconjugates onto screen-printed carbon electrodes, quantification of Cp was accomplished by DPV measurement of 1-naphthol generated after 1-naphthylphosphate addition. Linear ranges of calibration curves and detection limits were 0.1–1000 µg/mL and 0.040 µg/mL (Protein A-MBs), and 0.025–20 µg/mL and 0.018 µg/mL (Strept-MBs). Good results were obtained in the determination of Cp in spiked human serum samples.


Item Type:Article
Uncontrolled Keywords:Ceruloplasmin, Electrochemical magnetic immunosensor, Human serum
Subjects:Sciences > Chemistry > Analytic chemistry
ID Code:44742
Deposited On:20 Sep 2017 11:44
Last Modified:05 Aug 2019 08:51

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