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Viologen-functionalized single-walled carbon nanotubes as carrier nanotags for electrochemical immunosensing. Application to TGF-β1 cytokine

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Sánchez Tirado, Esther and Arellano, Luis M. and González Cortés, Araceli and Yáñez Sedeño, Paloma and Langa, Fernando and Pingarrón Carrazon, José Manuel (2017) Viologen-functionalized single-walled carbon nanotubes as carrier nanotags for electrochemical immunosensing. Application to TGF-β1 cytokine. Biosensors and Biolelectrics, 98 . pp. 240-247. ISSN 0956-5663(Print) , 1873-4235 (Online)

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Official URL: http://www.sciencedirect.com/science/article/pii/S0956566317304487?via%3Dihub



Abstract

Viologen-SWCNT hybrids are synthesized by aryl-diazonium chemistry in the presence of isoamyl nitrite followed by condensation reaction of the resulting HOOC-PheSWCNT with 1-(3-aminoethyl)-4,4’-bipyridinium bromine and N-alkylation with 2- bromoethylamine. The V-Phe-SWCNT hybrids were characterized by using different spectroscopic techniques (FT-IR, Raman, UV-vis), TGA and Kaiser test. ViologenSWCNTs were used for the preparation of an electrochemical immunosensor for the determination of the transforming growth factor β1 (TGF-β1) cytokine considered as a reliable biomarker in several human diseases. The methodology involved preparation of VPhe-SWCNT(-HRP)-anti-TGF conjugates by covalent linkage of HRP and anti-TGF onto V-Phe-SWCNT hybrids. Biotinylated anti-TGF antibodies were immobilized onto 4- carboxyphenyl-functionalized SPCEs modified with streptavidin and a sandwich type 2 immunoassay was implemented for TGF-β1 with signal amplification using V-PheSWCNT(-HRP)-anti-TGF conjugates as carrier tags. The analytical characteristics exhibited by the as prepared immunosensor (range of linearity between 2.5 and 1000 pg mL-1 TGF-β1; detection limit of 0.95 pg mL-1 ) improve notably those reported with other previous immunosensors or ELISA kits. A great selectivity against other proteins was also found. The prepared immunosensor was validated by determining TGF-β1 in real saliva samples. Minimal sample treatment was required and the obtained results were in excellent agreement with those obtained by using a commercial ELISA kit.


Item Type:Article
Uncontrolled Keywords:Viologen, Transforming growth factor β1, Cytokine Saliva Electrochemical immunosensor
Subjects:Sciences > Chemistry > Analytic chemistry
ID Code:45120
Deposited On:17 Oct 2017 09:05
Last Modified:13 Mar 2018 11:02

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