Publication: Efecto individual y conjunto de la administración de Dexametasona y Kanamicina sobre "Rattus Norvegicus"
Loading...
Official URL
Full text at PDC
Publication Date
2016
Authors
Advisors (or tutors)
Editors
Journal Title
Journal ISSN
Volume Title
Publisher
Citations
Exportar
Abstract
En este trabajo se analizaron los efectos del tratamiento con el glucocorticoide dexametasona por vía subcutánea y con el antibiótico kanamicina por vía oral, tanto individualmente como de forma conjunta, para valorar las posibles interacciones. Los sujetos experimentales fueron ratas jóvenes macho (Rattus norvegicus), estirpe Wistar, divididos en cuatro grupos: control, dexametasona, kanamicina y doble tratamiento (dexametasona y kanamicina). Se valoró la progresión del peso corporal; los pesos e índices organosomáticos del hígado, bazo, timo y glándulas suprarrenales; el efecto inmunosupresor sobre la pulpa blanca y la proporción de IgGs plasmáticas; el glucógeno hepático; la glucosa y las proteínas plasmáticas; la presencia de glucosuria y cetonuria; la microbiota gastrointestinal; y el cariotipo. Los resultados mostraron que la dexametasona disminuyó el peso corporal, provocó una atrofia del timo y glándulas suprarrenales, así como una disminución del tejido linfoide esplénico. Además, aumentó el tamaño del hígado y provocó hiperglucemia y glucosuria. La kanamicina sólo mostró efectos a nivel gastrointestinal, reduciendo las poblaciones de microorganismos aerobios mesófilos y enterobacterias, mostrando tendencia a reducir los enterococos. Este antibiótico no mostró ningún otro efecto ni interacción significativa con la dexametasona, probablemente por su casi nula absorción gastrointestinal. No hubo efectos sobre el cariotipo.
In this study, the efects of subcutaneus administration of glucocorticoid dexamethasone and oraly given antibiotic kanamycin were analyzed, individually and together, due to value possible interactions between them. The experimental subjects were male young rats (Rattus norvegicus), Wistar strain, divided in four groups: control, dexamethasone, kanamycin and double treatment (dexamethasone and kanamycin). The body weight progression in time was evaluated as well as the weight and organosomatic-index of the liver, spleen, thymus and the adrenal glands; Also we investigated the immunosuppressive effect on the white pulp of spleen and on the serum levels of IgG, the effects on the liver glycogen, serum glucose levels and plasmatic proteins, the presence of glycosuria and ketonuria, and some possible disorders in the gastrointestinal microbiota and on the karyotype. The results showed that dexamethasone decreased body weight, and it caused atrophy of the thymus and adrenal glands with a decreased of the splenic lymphoid tissue. In addition, it increased liver size and 3 caused hyperglycemia and glycosuria. Kanamycin showed only gastrointestinal effects with a decrease of the population of mesophilic aerobic microflora and gastrointestinal enterobacters, and some tendency to reduce the enterococcus. This antibiotic showed no other effect or significant interaction with dexamethasone, probably because of its minimal gastrointestinal absorption. There was no effect on the karyotype.
In this study, the efects of subcutaneus administration of glucocorticoid dexamethasone and oraly given antibiotic kanamycin were analyzed, individually and together, due to value possible interactions between them. The experimental subjects were male young rats (Rattus norvegicus), Wistar strain, divided in four groups: control, dexamethasone, kanamycin and double treatment (dexamethasone and kanamycin). The body weight progression in time was evaluated as well as the weight and organosomatic-index of the liver, spleen, thymus and the adrenal glands; Also we investigated the immunosuppressive effect on the white pulp of spleen and on the serum levels of IgG, the effects on the liver glycogen, serum glucose levels and plasmatic proteins, the presence of glycosuria and ketonuria, and some possible disorders in the gastrointestinal microbiota and on the karyotype. The results showed that dexamethasone decreased body weight, and it caused atrophy of the thymus and adrenal glands with a decreased of the splenic lymphoid tissue. In addition, it increased liver size and 3 caused hyperglycemia and glycosuria. Kanamycin showed only gastrointestinal effects with a decrease of the population of mesophilic aerobic microflora and gastrointestinal enterobacters, and some tendency to reduce the enterococcus. This antibiotic showed no other effect or significant interaction with dexamethasone, probably because of its minimal gastrointestinal absorption. There was no effect on the karyotype.
Description
Grado en Biología, Mención Biosanitaria, Curso 2015-2016