Universidad Complutense de Madrid
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Mesoporous core-shell silica nanoparticles with anti-fouling properties for ovarian cancer therapy

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Sánchez Salcedo, Sandra y Vallet Regí, María y Allaf Shanin, Sophia y Glackin, Carlotta A. y Zinck, Jeffrey I. (2018) Mesoporous core-shell silica nanoparticles with anti-fouling properties for ovarian cancer therapy. Chemical Engineering Journal . ISSN 1385-8947

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URL Oficial: https://www.elsevier.com/




Resumen

Mesoporous silica nanoparticles (MSNPs) have many potential applications in biomedical fields. However, when MSNPs are exposed to plasma, protein adsorption leads to opsonization and decreases blood circulation time. A new multifunctional nanodevice based on polyethylenimine (PEI) coated core-shell Fe⁠3O⁠4@SiO⁠2 MSNPs with a zwitterionic 2-methacryloyloxyethyl phosphorylcholine (MPC) surface was designed to minimize unspecific protein adhesion. Particle size measurements demonstrated an excellent non-fouling capacity in solutions containing Bovine Serum Albumin (BSA) and Fetal Bovine Serum (FBS) plasma proteins. The system was used in this study to co-deliver two different cargos: siRNA and daunorubicin. Anti-TWIST siRNA plays critical role in modulating knockdown of TWIST and sensitizing cells to chemotherapeutics such as daunorubicin for ovarian cancer therapy. The drug was released in response to externally controlled oscillating magnetic fields (OMF).
siRNA (siGFP) silenced expression of green fluorescence protein (GFP) in Ovcar8 cancer cells, demonstrating the incorporation of core shell MSNPs into cells and siGFP delivery. The synergistic effect of the co-release of anti-TWIST-siRNA loaded in the PEI and daunorubicin loaded in NPs’ pores caused increased cytotoxicity in Ovcar8 of up to 50% from both zwitteronic and non-zwitteronic NPs. The system is the first example of silencing by anti-TWITS-siRNA/daunorubicin co-delivered using zwitterionic core-shell nanoparticles with low-fouling adsorption. This engineered multifunctional approach may provide therapeutic potential for the treatment of currently incurable ovarian cáncer.


Tipo de documento:Artículo
Información Adicional:

RESEARCHER ID N-4501-2014 (Sandra Sánchez Salcedo)
ORCID 0000-0002-1889-2057 (Sandra Sánchez Salcedo)
RESEARCHER ID M-3378-2014 (María Vallet Regí)
ORCID 0000-0002-6104-4889 (María Vallet Regí)

Palabras clave:Zwitterion, PEI core-shell MSNPs, Co-delivery, siRNA, Daunorubicin, Cancer therapy
Materias:Ciencias > Química > Materiales
Ciencias > Química > Química inorgánica
Código ID:45938
Depositado:05 Feb 2018 18:30
Última Modificación:06 Feb 2018 11:50

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