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Determination of ibuprofen enantiomers in breast milk using vortex-assisted matrix solid-phase dispersion and direct chiral liquid chromatography

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León González, María Eugenia and Rosales Conrado, Noelia (2017) Determination of ibuprofen enantiomers in breast milk using vortex-assisted matrix solid-phase dispersion and direct chiral liquid chromatography. Journaol of Chromatography A, 1514 . pp. 88-94. ISSN 0021-9673, 1873-3778

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Official URL: http://www.elsevier.com/locate/chroma



Abstract

A mixture of B-cyclodextrin (B-CD) and primary and secondary amine (PSA) sorbents was employed for the extraction and quantification of ibuprofen enantiomers from human breast milk, combining a vortex-assisted matrix solid-phase dispersion method (MSPD) and direct chiral liquid chromatography (CLC) with ultraviolet detection (UV). The MSPD sample preparation procedure was optimized focusing on both the type and amount of dispersion/sorption sorbents and the nature of the elution solvent, in order to obtain acceptable recoveries and avoiding enantiomer conversion. These MSPD parameters were optimized with the aid of an experimental design approach. Hence, a factorial design was used for identification of the main variables affecting the extraction process of ibuprofen enantiomers. Under optimum selected conditions, MSPD combined with direct CLC-UV was successfully applied for ibuprofen enantiomeric determination in breast milk at enantiomer levels between 0.15 and 6.0 µg∙g-1. The proposed analytical method also provided good repeatability, with relative standard deviations of 6.4 % and 8.3 % for the intra-day and inter-day precision, respectively.


Item Type:Article
Uncontrolled Keywords:Matrix solid-phase dispersion, Vortex, Ibuprofen Enantiomers, Breast milk, Direct chiral liquid chromatography
Subjects:Sciences > Chemistry > Analytic chemistry
ID Code:46022
Deposited On:15 Jan 2018 09:35
Last Modified:01 Oct 2019 23:01

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