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In vitro equine embryo production using air-dried spermatozoa, with different activation protocols and culture systems

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Alonso Gómez, Ángel Luis and Castex, C. Baca and Ferrante, A. and Pinto, M. and Castañeira, C. and Trasorras, V. and Gambarotta, M. C. and Losinno, L. and Miragaya, M. (2015) In vitro equine embryo production using air-dried spermatozoa, with different activation protocols and culture systems. Andrologia, 47 . pp. 387-394. ISSN 0303-4569

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Official URL: http://onlinelibrary.wiley.com/journal/10.1111/(ISSN)1439-0272



Abstract

The aim of this work was to evaluate the use of air-dried spermatozoa for in vitro production of equine embryos and verify if sperm extract activation and in vivo culture improve in vitro embryo production. Cooled spermatozoa (con-trol) and air-dried spermatozoa stored for 2, 14 or 28 days were used for ICSI sperm extract, or ionomycin was used for oocyte activation, and embryos were in vitro or in vivo (in mare0s oviduct) cultured for 7 days. With in vitro cul-ture, cleavage rate was higher when activating with sperm extract (P < 0.05). No differences in embryo development were seen between the two activation treatments nor between storage periods (P > 0.05). Blastocysts were obtained with cooled spermatozoa, and morulae were achieved using in vivo culture with 28-day storage spermatozoa and ionomycin-activated oocytes. When in vivo culture was performed, sperm DNA fragmentation was assessed using the sperm chromatin dispersion test and did not show statistical correlation with cleavage nor embryo recovery rates. In conclusion, equine embryos can be pro-duced using air-dried spermatozoa stored for several weeks. Sperm extract acti-vation increased cleavage rates but did not improve embryo development. In vivo culture allowed intrauterine stage embryos to be achieved.


Item Type:Article
Uncontrolled Keywords:Air-dried spermatozoa; equine; ICSI; in vivo culture; sperm extract
Subjects:Medical sciences > Biology > Animal physiology
ID Code:46370
Deposited On:07 Feb 2018 15:48
Last Modified:07 Feb 2018 15:48

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