Universidad Complutense de Madrid
E-Prints Complutense

Human amniotic membrane as newly identifed source of amniotic fuid pulmonary surfactant

Impacto

Downloads

Downloads per month over past year

Lemke, Angela and Castillo-Sánchez, José Carlos and Prodinger, Florian and Ceranic, Asja and Hennerbichler-Lugscheider, Simone and Pérez Gil, Jesús and Redl, Heinz and Wolbank, Susanne (2017) Human amniotic membrane as newly identifed source of amniotic fuid pulmonary surfactant. Scientific Reports, 7 (6406). pp. 1-13. ISSN 2045-2322, ESSN: 2045-2322

[img]
Preview
PDF
Creative Commons Attribution.

5MB

Official URL: https://www.nature.com/articles/s41598-017-06402-w



Abstract

Pulmonary surfactant (PS) reduces surface tension at the air-liquid interface in the alveolar epithelium of the lung, which is required for breathing and for the pulmonary maturity of the developing foetus. However, the origin of PS had never been thoroughly investigated, although it was assumed to be secreted from the foetal developing lung. Human amniotic membrane (hAM), particularly its epithelial cell layer, composes the amniotic sac enclosing the amniotic fuid. In this study, we therefore aimed to investigate a potential contribution of the cellular components of the hAM to pulmonary surfactant found in amniotic fuid. We identifed that cells within the native membrane contain lamellar bodies and express all four surfactant proteins as well as ABCA3. Lipidomic profling by nanoESI – MS/MS revealed the presence of the essential lipid species as found in PS. Also, the biophysical activity of conditioned cell culture supernatant obtained from hAM was tested with captive bubble surfactometry. hAM supernatant showed the ability to reduce surface tension, similar to human PS obtained from bronchoalveolar lavage. This means that hAM produces the essential PS-associated components and can therefore contribute as second potential source of PS in amniotic fuid aside from the foetal lung.


Item Type:Article
Uncontrolled Keywords:Amniotic membrane; Pulmonary surfactant; Cell biology
Subjects:Medical sciences > Biology > Molecular biology
Medical sciences > Biology > Biochemistry
ID Code:46515
Deposited On:20 Feb 2018 11:51
Last Modified:21 Feb 2018 09:22

Origin of downloads

Repository Staff Only: item control page