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Molecular genetics of naringenin biosynthesis, a typical plant secondary metabolite produced by Streptomyces clavuligerus

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Álvarez Álvarez, Jesús and Botas Muñoz, Alma María and Albillos, Silvia M. and Rumbero, Ángel and Martín, Juan F. and Liras, Paloma (2015) Molecular genetics of naringenin biosynthesis, a typical plant secondary metabolite produced by Streptomyces clavuligerus. Microbial Cell Factories, 14 (1). pp. 1-12. ISSN ESSN: 1475-2859

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Official URL: https://microbialcellfactories.biomedcentral.com/articles/10.1186/s12934-015-0373-7



Abstract

Background: Some types of flavonoid intermediates seemed to be restricted to plants. Naringenin is a typical plant metabolite, that has never been reported to be produced in prokariotes. Naringenin is formed by the action of a chal cone synthase using as starter 4-coumaroyl-CoA, which in dicotyledonous plants derives from phenylalanine by the action of a phenylalanine ammonia lyase.
Results: A compound produced by Streptomyces clavuligerus has been identified by LC–MS and NMR as naringenin and coelutes in HPLC with a naringenin standard. Genome mining of S. clavuligerus revealed the presence of a gene for a chalcone synthase (ncs), side by side to a gene encoding a P450 cytochrome (ncyP) and separated from a gene encoding a Pal/Tal ammonia lyase (tal). Deletion of any of these genes results in naringenin non producer mutants. Complementation with the deleted gene restores naringenin production in the transformants. Furthermore, narin genin production increases in cultures supplemented with phenylalanine or tyrosine.
Conclusion: This is the first time that naringenin is reported to be produced naturally in a prokariote. Interestingly three non-clustered genes are involved in naringenin production, which is unusual for secondary metabolites. A ten tative pathway for naringenin biosynthesis has been proposed.


Item Type:Article
Uncontrolled Keywords:Naringenin; Secondary metabolism; Streptomyces clavuligerus; Histidine/phenylalanine ammonium lyase
Subjects:Medical sciences > Biology > Microbiology
ID Code:46648
Deposited On:28 Feb 2018 13:20
Last Modified:28 Feb 2018 13:20

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