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Mycoflora isolation and molecular characterization of Aspergillus and Fusarium species in Tunisian cereals

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Jedidi, Ines and Soldevilla, Carlos and Lahouar, Amani and Marín, Patricia and González Jaén, María Teresa and Said, Salem (2017) Mycoflora isolation and molecular characterization of Aspergillus and Fusarium species in Tunisian cereals. Saudi Journal of Biological Sciences . ISSN 1319-562X

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Official URL: https://www.sciencedirect.com/science/article/pii/S1319562X1730325X?via%3Dihub



Abstract

Wheat, barley and maize are the mainly consumed cereals in Tunisia. This study aimed to determine the mycoflora of these cereals with special focus on the mycotoxigenic Aspergillus and Fusarium species. Freshly harvested samples and other stored samples of each type of cereal (31 and 34 samples, respectively) were collected in Tunisia and cultured for fungal isolation and identification. Identification of fungal genera was based on morphological features. Aspergillus and Fusarium species were identified by species specific PCR assays complemented with DNA sequencing. Alternaria (70.83%), Eurotium (62.50%), Aspergillus (54.17%) and Penicillium (41.67%) were the most frequent fungi isolated from wheat. Penicillium (75%), Aspergillus (70%), Eurotium (65%) and Alternaria (65%) were the most frequently recovered genera from barley. The predominant genera in maize were Aspergillus(76.19%), Eurotium (42.86%), and Penicillium (38.09%). Aspergilllus, Penicillium, Fusarium and Alternaria were detected in both stored and freshly harvested grain samples. The frequencies of contamination with Aspergillus, Fusarium and Alternaria were higher in freshly harvested samples, whereas Penicillium species were more frequent in stored samples. The predominant Aspergillus species detected were A. flavus and A. niger. The Fusarium species detected were F. equiseti, F. verticillioides, F. nygamai, and F. oxysporum. This study suggested the potential risk for Aflatoxins and, to a lesser extent, for Ochratoxin A in Tunisian cereals. This is the first survey about mycoflora associated with wheat, barley and maize in Tunisia.


Item Type:Article
Uncontrolled Keywords:Mycoflora; Cereal; Aspergillus; Fusarium; DNA-based method; Tunisia
Subjects:Medical sciences > Biology
Medical sciences > Biology > Genetics
ID Code:46819
Deposited On:09 Mar 2018 18:17
Last Modified:10 Dec 2018 15:30

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