Universidad Complutense de Madrid
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Determination of B-N-oxalyl-L-a, B-diaminopropionic acid and homoarginine in Lathyrus sativus and Lathyrus cicera by capillary zone electrophoresis

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Sacristán San Cristóbal, Mara y Varela, Alejandro y Martín Pedrosa, Mercedes y Burbano, Carmen y Cuadrado Vives, María del Carmen y Legaz González, María Estrella y Muzquiz, Mercedes (2015) Determination of B-N-oxalyl-L-a, B-diaminopropionic acid and homoarginine in Lathyrus sativus and Lathyrus cicera by capillary zone electrophoresis. Journal of the Science of Food and Agriculture, 95 (7). pp. 1414-1420. ISSN 0022-5142, ESSN: 1097-0010

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URL Oficial: https://onlinelibrary.wiley.com/doi/abs/10.1002/jsfa.6792



Resumen

BACKGROUND: Lathyrus species as legumes represent an alternative protein source for human and animal nutrition. Heavy consumption of these species can lead to lathyrism, caused by the non-protein amino acid B-N-oxalyl-L-a, B-diaminopropionic acid (B-ODAP). Currently, there is no well-defined level below which B-ODAP is considered non-toxic. In this work, the B-ODAP content was determined in L. sativus and L. cicera samples to assess their potential toxicity. Homoarginine is another non-protein amino acid found in Lathyrus spp. with interesting implications for human and animal nutrition.
RESULTS: The level of B-ODAP found in these two species ranged from 0.79 to 5.05 mg g−1. The homoarginine content of the samples ranged from 7.49 to 12.44 mg g−1.
CONCLUSION: This paper describes an accurate, fast and sensitive method of simultaneous detection and quantification of B-ODAP and homoarginine by capillary zone electrophoresis in L. cicera and L. sativus seeds. Moreover, several methods of extraction were compared to determine the highest performance.


Tipo de documento:Artículo
Palabras clave:B-ODAP; Capillary zone electrophoresis; Homoarginine; Neurolathyrism; Lathyrus sativus; Lathyrus cicera
Materias:Ciencias Biomédicas > Biología > Botánica
Ciencias Biomédicas > Biología > Fisiología vegetal
Código ID:47140
Depositado:12 Abr 2018 07:24
Última Modificación:10 May 2018 08:26

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