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Determination of B-N-oxalyl-L-a, B-diaminopropionic acid and homoarginine in Lathyrus sativus and Lathyrus cicera by capillary zone electrophoresis

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Sacristán San Cristóbal, Mara and Varela, Alejandro and Martín Pedrosa, Mercedes and Burbano, Carmen and Cuadrado Vives, María del Carmen and Legaz González, María Estrella and Muzquiz, Mercedes (2015) Determination of B-N-oxalyl-L-a, B-diaminopropionic acid and homoarginine in Lathyrus sativus and Lathyrus cicera by capillary zone electrophoresis. Journal of the Science of Food and Agriculture, 95 (7). pp. 1414-1420. ISSN 0022-5142, ESSN: 1097-0010

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Official URL: https://onlinelibrary.wiley.com/doi/abs/10.1002/jsfa.6792



Abstract

BACKGROUND: Lathyrus species as legumes represent an alternative protein source for human and animal nutrition. Heavy consumption of these species can lead to lathyrism, caused by the non-protein amino acid B-N-oxalyl-L-a, B-diaminopropionic acid (B-ODAP). Currently, there is no well-defined level below which B-ODAP is considered non-toxic. In this work, the B-ODAP content was determined in L. sativus and L. cicera samples to assess their potential toxicity. Homoarginine is another non-protein amino acid found in Lathyrus spp. with interesting implications for human and animal nutrition.
RESULTS: The level of B-ODAP found in these two species ranged from 0.79 to 5.05 mg g−1. The homoarginine content of the samples ranged from 7.49 to 12.44 mg g−1.
CONCLUSION: This paper describes an accurate, fast and sensitive method of simultaneous detection and quantification of B-ODAP and homoarginine by capillary zone electrophoresis in L. cicera and L. sativus seeds. Moreover, several methods of extraction were compared to determine the highest performance.


Item Type:Article
Uncontrolled Keywords:B-ODAP; Capillary zone electrophoresis; Homoarginine; Neurolathyrism; Lathyrus sativus; Lathyrus cicera
Subjects:Medical sciences > Biology > Botany
Medical sciences > Biology > Plant physiology
ID Code:47140
Deposited On:12 Apr 2018 07:24
Last Modified:09 Apr 2019 09:39

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