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Characterisation of the porcine eyeball as an in-vitro model for dry eye

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Meduni, Francesco and Davies, Leon N. and Madrid Costa, David and Fratini, Antonio and Wolffsohn, James S. (2018) Characterisation of the porcine eyeball as an in-vitro model for dry eye. Contact Lens and Anterior Eye, 41 (1). pp. 13-17. ISSN 1367-0484

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Official URL: https://doi.org/10.1016/j.clae.2017.09.003


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Abstract

Purpose: To characterise the anatomical parameters of the porcine eye for potentially using it as a laboratory model of dry eye.
Methods: Anterior chamber depth and angle, corneal curvature, shortest and longest diameter, endothelial cell density, and pachymetry were measured in sixty freshly enucleated porcine eyeballs.
Results: Corneal steepest meridian was 7.85 ± 0.32 mm, corneal flattest meridian was 8.28 ± 0.32 mm, shortest corneal diameter was 12.69 ± 0.58 mm, longest corneal diameter was 14.88 ± 0.66 mm and central corneal ultrasonic pachymetry was 1009 ± 1 μm. Anterior chamber angle was 28.83 ± 4.16°, anterior chamber depth was 1.77 ± 0.27 mm, and central corneal thickness measured using OCT was 1248 ± 144 μm. Corneal endothelial cell density was 3250 ± 172 cells/mm2.
Conclusions: Combining different clinical techniques produced a pool of reproducible data on the porcine eye anatomy, which can be used by researchers to assess the viability of using the porcine eye as an in-vitro/ex-vivo model for dry eye. Due to the similar morphology with the human eye, porcine eyeballs may represent a useful and cost effective model to individually study important key factors in the development of dry eye, such as environmental and mechanical stresses.


Item Type:Article
Additional Information:

Received 13 July 2017, Revised 28 August 2017, Accepted 8 September 2017, Available online 3 October 2017.

Uncontrolled Keywords:Porcine eye; In vitro; Ex vivo; Dry eye; Confocal microscopy; Optical coherence tomography
Subjects:Medical sciences > Medicine > Ophtalmology
Medical sciences > Optics > Optometry
Medical sciences > Optics > Physiological optics
ID Code:47264
Deposited On:27 Apr 2018 10:11
Last Modified:27 Apr 2018 12:08

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