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Endocrine disruptors in soil: effects of bisphenol A on gene expression of the earthworm Eisenia fetida

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Novo Rodríguez, Marta and Verdú, I. and Trigo Aza, María Dolores and Martínez-Guitarte, J. L. (2018) Endocrine disruptors in soil: effects of bisphenol A on gene expression of the earthworm Eisenia fetida. Ecotoxicology and Environmental Safety, 150 . pp. 159-167. ISSN 0147-6513, ESSN: 1090-2414

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Official URL: https://www.sciencedirect.com/science/article/pii/S0147651317308655



Abstract

Xenobiotics such as bisphenol A (BPA), are present in biosolids, which are applied as organic fertilizers in agricultural fields. Their effects on soil life have been poorly assessed, and this is particularly important in the case of earthworms, which represent the main animal biomass in this medium. In the present work we study the impacts of BPA on gene expression of Eisenia fetida, a widely used ecotoxicological model. Chronic soil tests and acute contact tests were performed, and gene expression was analyzed in total tissue and in masculine reproductive organs of the earthworms. The genes studied in this research played a role in endocrine pathways, detoxification mechanisms, stress response, epigenetics, and genotoxicity. Most of the genes were identified for the first time, providing potentially useful biomarkers for future assessments. For chronic exposures, no changes were detected in whole-body tissue; however, masculine reproductive organs showed changes in the expression of genes related to endocrine function (EcR, MAPR, AdipoR), epigenetic mechanisms (DNMTs), genotoxicity (PARP1), and stress responses (HSC70 4). For acute exposures, the expression of one epigenetic-related gene was altered for both whole-body tissues and male reproductive organs (Piwi2). Further changes were detected for whole-body tissues involved in detoxification (Metallothionein), stress (HSC70 4), and genotoxicity (PARP1) mechanisms. Acute exposure effects were also tested in whole-body tissues of juveniles, showing changes in the expression of Metallothionein and Piwi2. The molecular changes found in the analyzed earthworms indicate that exposure to BPA may have negative implications in their populations. Particularly interesting are the alterations related to epigenetic mechanisms, which suggest that future generations may be impacted. This study is the first to evaluate the molecular effects of BPA on soil organisms, and further assays will be necessary to better characterize the true environmental repercussions. Capsule: Levels of gene expression in total-body tissues and masculine reproductive organs were analyzed in earthworms after exposure to bisphenol A and we observed associated changes in detoxification, endocrine, epigenetic, genotoxic and stress pathways.


Item Type:Article
Uncontrolled Keywords:Xenobiotics; Annelids; Epigenetics; Masculine tissue; Biomarkers; Bisphenol A
Subjects:Medical sciences > Biology > Invertebrates
ID Code:47334
Deposited On:26 Apr 2018 08:51
Last Modified:10 Dec 2018 15:25

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