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Regional chemoarchitecture of the brain of lungfishes based on calbindin D-28K and calretinin immunohistochemistry

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Morona Arribas, Ruth and López Redondo, Jesús María and Northcutt, R. Glenn and González Gallegos, Agustín (2018) Regional chemoarchitecture of the brain of lungfishes based on calbindin D-28K and calretinin immunohistochemistry. Journal of Comparative Neurology, 526 (9). pp. 1457-1497. ISSN 0021-9967, ESSN: 1096-9861

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Official URL: https://onlinelibrary.wiley.com/doi/abs/10.1002/cne.24422



Abstract

Lungfishes are the closest living relatives of land vertebrates, and their neuroanatomical organization is particularly relevant for deducing the neural traits that have been conserved, modified, or lost with the transition from fishes to land vertebrates. The immunohistochemical localization of calbindin (CB) and calretinin (CR) provides a powerful method for discerning segregated neuronal populations, fiber tracts, and neuropils and is here applied to the brains of Neoceratodus and Protopterus, representing the two extant orders of lungfishes. The results showed abundant cells containing these proteins in pallial and subpallial telencephalic regions, with particular distinct distribution in the basal ganglia, amygdaloid complex, and septum. Similarly, the distribution of CB and CR containing cells supports the division of the hypothalamus of lungfishes into neuromeric regions, as in tetrapods. The dense concentrations of CB and CR positive cells and fibers highlight the extent of the thalamus. As in other vertebrates, the optic tectum is characterized by numerous CB positive cells and fibers and smaller numbers of CR cells. The so-called cerebellar nucleus contains abundant CB and CR cells with long ascending axons, which raises the possibility that it could be homologized to the secondary gustatory nucleus of other vertebrates. The corpus of the cerebellum is devoid of CB and CR and cells positive for both proteins are found in the cerebellar auricles and the octavolateralis nuclei. Comparison with other vertebrates reveals that lungfishes share most of their features of calcium binding protein distribution with amphibians, particularly with salamanders.


Item Type:Article
Uncontrolled Keywords:Brain evolution; Calcium binding proteins; Choline acetyltransferase; Dipnoans; Segmentation; Tyrosine hydroxylase
Subjects:Medical sciences > Biology > Animal physiology
Medical sciences > Biology > Neurosciences
Medical sciences > Biology > Fishes
ID Code:47654
Deposited On:22 May 2018 07:47
Last Modified:22 May 2018 08:31

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