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Development of a Fluorescent Bodipy Probe for Visualization of the Serotonin 5-HT1A Receptor in Native Cells of the Immune System

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Hernández Torres, Gloria and Enríquez Palacios, Ernesto and Mecha Rodríguez, Miriam and Feliú Martínez, Ana and Rueda Zubiaurre, Ainoa and Angelina Querencias, Alba and Martín Cruz, Leticia and Martín Fontecha, María del Mar and Palomares, Oscar and Guaza Rodríguez, Carmen and Peña Cabrera, Eduardo and López Rodríguez, María L. and Ortega Gutiérrez, Silvia (2018) Development of a Fluorescent Bodipy Probe for Visualization of the Serotonin 5-HT1A Receptor in Native Cells of the Immune System. Bioconjugate Chemistry, 29 (6). pp. 2021-2027. ISSN 1043-1802

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Official URL: http://dx.doi.org/10.1021/acs.bioconjchem.8b00228




Abstract

Serotonin (5-HT) modulates key aspects of the immune system. However, its precise function and the receptors involved in the observed effects have remained elusive. Among the different serotonin receptors, 5-HT1A plays an important role in the immune system given its presence in cells involved in both the innate and adaptive immune responses, but its actual levels of expression under different conditions have not been comprehensively studied due to the lack of suitable tools. To further clarify the role of 5-HT1A receptor in the immune system, we have developed a fluorescent small molecule probe that enables the direct study of the receptor levels in native cells. This probe allows direct profiling of the receptor expression in immune cells using flow cytometry. Our results show that important subsets of immune cells including human monocytes and dendritic cells express functional 5-HT1A and that its activation is associated with anti-inflammatory signaling. Furthermore, application of the probe to the experimental autoimmune encephalomyelitis model of multiple sclerosis demonstrates its potential to detect the specific overexpression of the 5-HT1A receptor in CD4+ T cells. Accordingly, the probe reported herein represents a useful tool whose use can be extended to study the levels of 5-HT1A receptor in ex vivo samples of different immune system conditions.


Item Type:Article
Additional Information:

The Supporting Information is available free of charge on the ACS Publications website at DOI: 10.1021/acs.bioconjchem.8b00228.
Received: March 30, 2018; Revised: May 4, 2018; Published: May 7, 2018

Uncontrolled Keywords:Fluorescent bodipy probe; Serotonin; 5-HT1A; inmune system; Multiple sclerosis; encpphelamyelitits
Subjects:Sciences > Chemistry > Biochemistry
Medical sciences > Medicine > Immunology
ID Code:48271
Deposited On:28 Jun 2018 09:17
Last Modified:02 Jul 2018 11:45

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