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Stichodactyla helianthus' de novo transcriptome assembly: Discovery of a new actinoporin isoform

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Rivera de Torre, Esperanza and Martínez del Pozo, Álvaro and Garb, Jessica E. (2018) Stichodactyla helianthus' de novo transcriptome assembly: Discovery of a new actinoporin isoform. Toxicon, 150 . pp. 105-114. ISSN 0041-0101

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Official URL: https://reader.elsevier.com/reader/sd/A00EC6856378E3AAD2BC321B6018F5887EAD3D93BFA7D22DF0423EF7D6B3546291E87AD7931D13613527D7763E7A0793



Abstract

Transcriptomic profiling of venom producing tissues from different animals is an effective approach for discovering new toxins useful in biotechnological and pharmaceutical applications, as well in evolutionary comparative studies of venomous animals. Stichodactyla helianthus is a Caribbean sea anemone which produces actinoporins as part of its toxic venom. This family of pore forming toxins is multigenic and at least two different isoforms, encoded by separate genes, are produced by S. helianthus. These isoforms, sticholysins I and II, share 93% amino acid identity but differ in their pore forming activity and act synergistically. This observation suggests that other actinoporin isoforms, if present in the venomous mixture, could offer an advantageous strategy to modulate whole venom activity. Using high-throughput sequencing we generated a de novo transcriptome of S. helianthus and determined the relative expression of assembled transcripts using RNA-Seq to better characterize components of this species' venom, focusing on actinoporin diversity. Applying this approach, we have discovered at least one new actinoporin variant from S. helianthus in addition to several other putative venom components.


Item Type:Article
Uncontrolled Keywords:Actinoporin; Anemone; Isoform; Toxin; Transcriptome
Subjects:Sciences > Chemistry > Molecular biology
Sciences > Chemistry > Biochemistry
ID Code:48340
Deposited On:29 Jun 2018 11:01
Last Modified:01 Sep 2019 23:01

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