Publication: Reconocimiento molecular basado en técnicas de RMN
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2018-09-04
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Universidad Complutense de Madrid
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Abstract
El reconocimiento molecular está presente en numerosos procesos vitales y relevantes para la salud y la enfermedad. El conocimiento de la comunicación intermolecular a nivel estructural es de especial interés para el entendimiento de numerosos procesos biológicos. De entre la gran variedad de técnicas aplicadas al reconocimiento molecular, la Resonancia Magnética Nuclear (RMN) proporciona información valiosa a diferentes niveles de los bio-complejos formados. Desde el comienzo, la RMN ha sido explotada hasta alcanzar límites insospechados. En esta tesis, diferentes técnicas, métodos y protocolos basados en RMN se han aplicado extensamente para estudiar una variedad de procesos de reconocimiento molecular de diferentes niveles de complejidad. En particular, esta aplicación de la RMN ha permitido recopilar información sobre las características del reconocimiento molecular de estos eventos abordado desde las perspectivas del ligando y/o receptor. Se ha hecho especial hincapié en desentrañar los fenómenos de interacción en los que intervienen los carbohidratos. De manera paralela, también se han utilizado métodos de RMN análogos en el campo de la dinámica de proteínas así como para cribar mezclas de ligandos mediante un enfoque de química dinámica combinatoria guiada por RMN...
Molecular recognition is at heart of life processes relevant to health and disease. Deciphering the code of the intermolecular communication at structural level is enormous interest for the understanding of many biological events. Among the variety of techniques applied in this field, NMR may provide valuable information, at different levels, on the formed bio-complexes. NMR has been continuously exploited to reach unexpected limits a few years ago. In this thesis, different NMR techniques, methods and protocols have been extensively applied to study a variety molecular recognition processes at diverse levels of complexity. In particular, these applications of NMR have permitted gathering information on the molecular recognition features of these events, from the ligand and/or receptor perspectives. Special emphasis has been paid to unravel interaction phenomena where carbohydrates are involved. In a parallel manner, analogous NMR methods have also been used in the field of protein dynamics as well as for screening ligand mixtures through a NMR-guided dynamic combinatorial chemistry approach...
Molecular recognition is at heart of life processes relevant to health and disease. Deciphering the code of the intermolecular communication at structural level is enormous interest for the understanding of many biological events. Among the variety of techniques applied in this field, NMR may provide valuable information, at different levels, on the formed bio-complexes. NMR has been continuously exploited to reach unexpected limits a few years ago. In this thesis, different NMR techniques, methods and protocols have been extensively applied to study a variety molecular recognition processes at diverse levels of complexity. In particular, these applications of NMR have permitted gathering information on the molecular recognition features of these events, from the ligand and/or receptor perspectives. Special emphasis has been paid to unravel interaction phenomena where carbohydrates are involved. In a parallel manner, analogous NMR methods have also been used in the field of protein dynamics as well as for screening ligand mixtures through a NMR-guided dynamic combinatorial chemistry approach...
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Tesis inédita de la Universidad Complutense de Madrid, Facultad de Ciencias Químicas, leída el 21-12-2017