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Induction of VEGF secretion from bone marrow stromal cell line (ST-2) by the dissolution products of mesoporous silica glass particles containing CuO and SrO

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Balasubramanian, Preethi and Salinas Sánchez, Antonio J. and Sánchez Salcedo, Sandra and Detsch, Rainer and Vallet Regí, María and Boccaccini, Aldo R. (2018) Induction of VEGF secretion from bone marrow stromal cell line (ST-2) by the dissolution products of mesoporous silica glass particles containing CuO and SrO. Journal of Non-Crystalline Solids, 500 . pp. 217-224. ISSN 0022-3093 (In Press)

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Abstract

Certain biomaterials are capable of inducing the secretion of Vascular Endothelial Growth Factor (VEGF) from cells exposed to their biochemical influence, which plays a vital role in stimulating angiogenesis. Looking for this capacity, in this study three porous glasses were synthesized and characterized. Glass compositions (in mol-%) were: 60SiO2–(36-2x)CaO–4P2O5–xCuO–xSrO with x=0, 1 or 2.5, respectively, for B60, CuSr-1 or CuSr-2.5 glasses. Cu2+ and Sr2+ ions were added because of the reported biological capabilities of Cu2+ as angiogenic stimulator and Sr2+ as osteogenic stimulator. The objective of this study was to determine the concentration of the glass particles that, being out of the cytotoxic range, could increase VEGF secretion. The viability of cultivated bone marrow stromal cells (ST-2) was assessed. The samples were examined with light microscopy (LM) after the histochemical staining for haematoxylin and eosin (HE). The biological activity of glasses was evaluated in terms of the influence of the Cu2+ and Sr2+ ions on the cells. The dissolution products of CuSr-1 and CuSr-2.5 produced the highest secretion of VEGF from ST-2 cells after 48h of incubation. The combination of Cu2+ and Sr2+ lays the foundation for engineering a bioactive glass than can lead to vascularized, functional bone tissue when used in bone regeneration applications.


Item Type:Article
Additional Information:

RESEARCHER ID M-3316-2014 (Antonio Salinas Sánchez)
ORCID 0000-0002-8408-3389 (Antonio Salinas Sánchez)
RESEARCHER ID N-4501-2014 (Sandra Sánchez Salcedo)
ORCID 0000-0002-1889-2057 (Sandra Sánchez Salcedo)
RESEARCHER ID M-3378-2014 (María Vallet Regí)
ORCID 0000-0002-6104-4889 (María Vallet Regí)

Uncontrolled Keywords:Bioactive glasses, Ion release, Mesoporosity, Bone marrow stromal cells, Angiogenesis
Subjects:Sciences > Chemistry > Materials
Medical sciences > Pharmacy > Inorganic chemistry
ID Code:49067
Deposited On:24 Sep 2018 08:15
Last Modified:24 Sep 2018 14:20

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