Publication: Papel de FIP1 en la regulación del desarrollo y la respuesta a estrés mediante poliadenilación alternativa en Arabidopsis thaliana
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2018-09-21
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Universidad Complutense de Madrid
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Abstract
Las plantas son organismos sésiles que deben adaptar su crecimiento al medio en que se encuentran. Para ello, han desarrollado una serie de mecanismos de respuesta a diferentes señales endógenas y exógenas que regulan la activación de diferentes rutas genéticas. La regulación de la expresión génica en eucariotas se da a distintos niveles, siendo el transcripcional el más estudiado. Sin embargo, los procesos de regulación postranscripcional tienen un papel muy relevante en la regulación de la expresión. Recientemente se ha observado que la poliadenilación de los tránscritos inmaduros es clave en la regulación de la expresión génica, ya que participa en los procesos de procesamiento postranscripcional, estabilidad, exportación nuclear y localización subcelular de los ARN mensajeros. Este nivel de regulación depende de la localización del sitio de corte y poliadenilación (PAS), que se selecciona gracias a la interacción de numerosas proteínas implicadas en el reconocimiento de secuencias y activación de la Poly(A) polimerasa. Este trabajo de tesis doctoral se centra en el análisis de la regulación de la expresión génica por poliadenilación alternativa (APA) en el organismo modelo Arabidopsis thaliana. Se han descrito diferentes tipos de APA que se han relacionado con diferentes tipos celulares, así como con su grado de diferenciación celular: Por un lado, la poliadenilación de diferentes puntos situados en el extremo 3’UTR (regiones canónicas) da lugar a mensajeros de diferente longitud, portadores de diferentes secuencias diana para proteínas reguladoras implicadas en la estabilidad y localización subcelular del mensajero maduro. Por otro lado, la poliadenilación de regiones no canónicas (extremo 5’UTR, CDS e intrones) se ha relacionado con sistemas de control de calidad de los mensajeros y con su procesamiento...
Plants are sessile organism that have to adapt their growth to the environment. They have developed several pathways in response to different endogen and exogen signals, so they can control the activation of different genetic routes. Eukaryotic regulation of gene expression takes place at different levels, being transcriptional level the most studied one. However, post-transcriptional regulation processes such as polyadenilation play an important role in gene expression regulation. It has been recently observed that the choice of Poly(A) Site (PAS) can effectively determine what the gene will code for and how it may be regulated, so it influences mRNA splicing, stability, transport and subcellular localization. This regulation level depends on the position of the Polyadenylation Site (PAS), which is selected through the interaction of several proteins that recognize specific sequences and induce activation of Poly(A) polymerase enzymes. This PhD work is focused in post-transcriptional regulation of gene expression through alternative polyadenylation (APA) in the model organism Arabidopsis thaliana. It has been described that different APA types are related to cell type specification and differentiation through different strategies: On one hand, polyadenylation of different 3’UTR positions (canonic regions) results in mRNA with different length that can present different regulatory sequences which have a role on stability and localization of mature mRNA. On the other hand, polyadenylation at non canonic regions (5’UTR, CDS and introns) has been related to quality mechanisms and splicing...
Plants are sessile organism that have to adapt their growth to the environment. They have developed several pathways in response to different endogen and exogen signals, so they can control the activation of different genetic routes. Eukaryotic regulation of gene expression takes place at different levels, being transcriptional level the most studied one. However, post-transcriptional regulation processes such as polyadenilation play an important role in gene expression regulation. It has been recently observed that the choice of Poly(A) Site (PAS) can effectively determine what the gene will code for and how it may be regulated, so it influences mRNA splicing, stability, transport and subcellular localization. This regulation level depends on the position of the Polyadenylation Site (PAS), which is selected through the interaction of several proteins that recognize specific sequences and induce activation of Poly(A) polymerase enzymes. This PhD work is focused in post-transcriptional regulation of gene expression through alternative polyadenylation (APA) in the model organism Arabidopsis thaliana. It has been described that different APA types are related to cell type specification and differentiation through different strategies: On one hand, polyadenylation of different 3’UTR positions (canonic regions) results in mRNA with different length that can present different regulatory sequences which have a role on stability and localization of mature mRNA. On the other hand, polyadenylation at non canonic regions (5’UTR, CDS and introns) has been related to quality mechanisms and splicing...
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Tesis inédita de la Universidad Complutense de Madrid, Facultad de Ciencias Biológicas, Departamento de Bioquímica y Biología Molecular, leída el 22 de Junio de 2017