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Applications of Nanomaterials Based on Magnetite and Mesoporous Silica on the Selective Detection of Zinc Ion in Live Cell Imaging

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Erami, Roghayeh Sadeghi and Ovejero, Karina and Meghdadi, Soraia and Filice, Marco and Amirnasr, Mehdi and Rodríguez Diéguez, Antonio and Orden, María Ulagares de la and Gómez Ruiz, Santiago (2018) Applications of Nanomaterials Based on Magnetite and Mesoporous Silica on the Selective Detection of Zinc Ion in Live Cell Imaging. Nanomaterials, 8 (6). 26 p.. ISSN 2079-4991

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Official URL: https://doi.org/10.3390/nano8060434




Abstract

Functionalized magnetite nanoparticles (FMNPs) and functionalized mesoporous silica nanoparticles (FMSNs) were synthesized by the conjugation of magnetite and mesoporous silica with the small and fluorogenic benzothiazole ligand, that is, 2(2-hydroxyphenyl)benzothiazole (hpbtz). The synthesized fluorescent nanoparticles were characterized by FTIR, XRD, XRF, 13C CP MAS NMR, BET, and TEM. The photophysical behavior of FMNPs and FMSNs in ethanol was studied using fluorescence spectroscopy. The modification of magnetite and silica scaffolds with the highly fluorescent benzothiazole ligand enabled the nanoparticles to be used as selective and sensitive optical probes for zinc ion detection. Moreover, the presence of hpbtz in FMNPs and FMSNs induced efficient cell viability and zinc ion uptake, with desirable signaling in the normal human kidney epithelial (Hek293) cell line. The significant viability of FMNPs and FMSNs (80% and 92%, respectively) indicates a potential applicability of these nanoparticles as in vitro imaging agents. The calculated limit of detections (LODs) were found to be 2.53 × 10−6 and 2.55 × 10−6 M for Fe3O4-H@hpbtz and MSN-Et3N-IPTMS-hpbtz-f1, respectively. FMSNs showed more pronounced zinc signaling relative to FMNPs, as a result of the more efficient penetration into the cells.


Item Type:Article
Additional Information:

Received: 7 May 2018 / Revised: 24 May 2018 / Accepted: 12 June 2018 / Published: 14 June 2018
(This article belongs to the Special Issue Nanostructured Biosensors)

Uncontrolled Keywords:nanomaterials, mesoporous silica, magnetite, live cell imaging, Zn2+ detection, Zn2+ sensors
Subjects:Sciences > Chemistry > Materials
Sciences > Chemistry > Chemistry, Organic
Medical sciences > Biology > Cytology
ID Code:49437
Deposited On:10 Oct 2018 13:05
Last Modified:11 Oct 2018 10:22

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