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Incorporation and effects of mesoporous SiO2-CaO nanospheres loaded with ipriflavone on osteoblast/osteoclast cocultures

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Casarrubios, Laura and Gómez-Cerezo, Natividad and Feito Castellano, María José, María José and Vallet Regí, María and Arcos Navarrete, Daniel and Portolés Pérez, María Teresa (2018) Incorporation and effects of mesoporous SiO2-CaO nanospheres loaded with ipriflavone on osteoblast/osteoclast cocultures. European Journal of Pharmaceutics and Biopharmaceutics, 133 . pp. 258-268. ISSN 0939-6411 (In Press)

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Abstract

Mesoporous nanospheres in the system SiO2-CaO (NanoMBGs) with a hollow core surrounded by a radial arrangement of mesopores were characterized, labeled with FITC (FITC-NanoMBGs) and loaded with ipriflavone (NanoMBG-IPs) in order to evaluate their incorporation and their effects on both osteoblasts and osteoclasts simultaneously and maintaining the communication with each other in coculture. The influence of these nanospheres on macrophage polarization towards pro-inflammatory M1 or reparative M2 phenotypes was also evaluated in basal and stimulated conditions through the expression of CD80 (as M1 marker) and CD206 (as M2 marker) by flow cytometry and confocal microscopy. NanoMBGs did not induce the macrophage polarization towards the M1 pro-inflammatory phenotype, favoring the M2 reparative phenotype and increasing the macrophage response capability against stimuli as LPS and IL-4. NanoMBG-IPs induced a significant decrease of osteoclast proliferat ion and resorption activity after 7 days in coculture with osteoblasts, without affecting osteoblast proliferation and viability. Drug release test demonstrated that only a fraction of the payload is released by diffusion, whereas the rest of the drug remains within the hollow core after 7 days, thus ensuring the local long-term pharmacological treatment beyond the initial fast IP release. All these data ensure an appropriate immune response to these nanospheres and the potential application of NanoMBG-IPs as local drug delivery system in osteoporotic patients.


Item Type:Article
Additional Information:

RESEARCHER ID M-3378-2014 (María Vallet Regí)
ORCID 0000-0002-6104-4889 (María Vallet Regí)
RESEARCHER ID L-6167-2014 (Daniel Arcos Navarrete)
ORCID 0000-0002-5367-7272 (Daniel Arcos Navarrete)
RESEARCHER ID U-1678-2017 (María Teresa Portolés Pérez)
ORCID 0000-0002-9681-0184 (María Teresa Portolés Pérez)

Uncontrolled Keywords:Mesoporous bioactive glasses; Nanospheres; Osteoblasts; Osteoclasts; Ipriflavone.
Subjects:Sciences > Chemistry > Materials
Medical sciences > Pharmacy > Inorganic chemistry
ID Code:49829
Deposited On:20 Nov 2018 11:31
Last Modified:23 Nov 2018 10:44

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